Analysis of macromolecular branched chain polypeptides by capillary electrophoresis and micellar electrokinetic chromatography

Miklós Idei, Gábor Dibó, Katalin Bogdán, Gábor Mezö, Anikó Horváth, Judit Érchegyi, György Mészáros, István Teplán, György Kéri, Ferenc Hudecz

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Amphoteric poly(Lys-[Glu1.0-DL-Ala4.1]), (EAK) and anionic poly(Lys-Ac-Glu0.98-DL-Ala3.98]), (AcEAK) branched chain polypeptides were analyzed by capillary electrophoresis (CE) and micellar elektrokinetic chromatography (MEKC) in the following buffers. A1: 0.25 N triethyl ammonium phosphate (TEAP) buffer (pH 2.25); A2: 100 mM sodium dodecyl sulfate (SDS) in buffer A1; B1: Na-borate buffer (pH 7.7); B2: 100 mM SDS in buffer B1; C1: Na-borate buffer (pH 11.0); C2: 100 mM SDS in buffer C1. Both EAK and AcEAK could be separated by a CE mechanism at pH 2.25 and by an MEKC mechanism at pH 11.0. Optimum results were achieved with CE in buffer A1 and with MEKC in buffer C2.

Original languageEnglish
Pages (from-to)1357-1360
Number of pages4
JournalELECTROPHORESIS
Volume17
Issue number8
DOIs
Publication statusPublished - Aug 1996

    Fingerprint

Keywords

  • Capillary electrophoresis
  • Macromolecular branched chain polypeptides
  • Micellar electrokinetic chromatography

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

Cite this