Analysis of linearly polarized fluorescence of chloroplasts oriented in polyacrylamide gel

A. O. Ganago, G. Garab, Á Faludi-Dániel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Linearly polarized fluorescence was measured in mesophyll chloroplasts of maize aligned in polyacrylamide gel. The dependence of fluorescence polarization on the squeezing parameter of the gel sample was studied at room temperature. Polarized fluorescence emission and polarization ratio spectra were recorded at low temperature (- 140°C). A theoretical model is proposed for a quantitative description of the polarized fluorescence emission and its dependence on the squeezing parameter. It is shown that chloroplasts are aligned as disc-shaped particles with no detectable deformation of their ultrastructure. The fluorescence emission and the polarization ratio spectra as well as the calculated orientation angles between the transition dipoles and the membrane plane were similar to those obtained with magnetically oriented chloroplasts.

Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalBBA - Bioenergetics
Volume723
Issue number2
DOIs
Publication statusPublished - May 27 1983

Fingerprint

Fluorescence Polarization
Chloroplasts
Fluorescence
Polarization
Temperature
Zea mays
Theoretical Models
Gels
Membranes
polyacrylamide gels

Keywords

  • (Maize)
  • Chloroplast orientation
  • Fluorescence
  • Polarization
  • Polyacrylamide gel

ASJC Scopus subject areas

  • Biophysics

Cite this

Analysis of linearly polarized fluorescence of chloroplasts oriented in polyacrylamide gel. / Ganago, A. O.; Garab, G.; Faludi-Dániel, Á.

In: BBA - Bioenergetics, Vol. 723, No. 2, 27.05.1983, p. 287-293.

Research output: Contribution to journalArticle

Ganago, A. O. ; Garab, G. ; Faludi-Dániel, Á. / Analysis of linearly polarized fluorescence of chloroplasts oriented in polyacrylamide gel. In: BBA - Bioenergetics. 1983 ; Vol. 723, No. 2. pp. 287-293.
@article{5e796a0dfe0f4f818e89b42c8b72b042,
title = "Analysis of linearly polarized fluorescence of chloroplasts oriented in polyacrylamide gel",
abstract = "Linearly polarized fluorescence was measured in mesophyll chloroplasts of maize aligned in polyacrylamide gel. The dependence of fluorescence polarization on the squeezing parameter of the gel sample was studied at room temperature. Polarized fluorescence emission and polarization ratio spectra were recorded at low temperature (- 140°C). A theoretical model is proposed for a quantitative description of the polarized fluorescence emission and its dependence on the squeezing parameter. It is shown that chloroplasts are aligned as disc-shaped particles with no detectable deformation of their ultrastructure. The fluorescence emission and the polarization ratio spectra as well as the calculated orientation angles between the transition dipoles and the membrane plane were similar to those obtained with magnetically oriented chloroplasts.",
keywords = "(Maize), Chloroplast orientation, Fluorescence, Polarization, Polyacrylamide gel",
author = "Ganago, {A. O.} and G. Garab and {\'A} Faludi-D{\'a}niel",
year = "1983",
month = "5",
day = "27",
doi = "10.1016/0005-2728(83)90128-7",
language = "English",
volume = "723",
pages = "287--293",
journal = "Biochimica et Biophysica Acta - Bioenergetics",
issn = "0005-2728",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Analysis of linearly polarized fluorescence of chloroplasts oriented in polyacrylamide gel

AU - Ganago, A. O.

AU - Garab, G.

AU - Faludi-Dániel, Á

PY - 1983/5/27

Y1 - 1983/5/27

N2 - Linearly polarized fluorescence was measured in mesophyll chloroplasts of maize aligned in polyacrylamide gel. The dependence of fluorescence polarization on the squeezing parameter of the gel sample was studied at room temperature. Polarized fluorescence emission and polarization ratio spectra were recorded at low temperature (- 140°C). A theoretical model is proposed for a quantitative description of the polarized fluorescence emission and its dependence on the squeezing parameter. It is shown that chloroplasts are aligned as disc-shaped particles with no detectable deformation of their ultrastructure. The fluorescence emission and the polarization ratio spectra as well as the calculated orientation angles between the transition dipoles and the membrane plane were similar to those obtained with magnetically oriented chloroplasts.

AB - Linearly polarized fluorescence was measured in mesophyll chloroplasts of maize aligned in polyacrylamide gel. The dependence of fluorescence polarization on the squeezing parameter of the gel sample was studied at room temperature. Polarized fluorescence emission and polarization ratio spectra were recorded at low temperature (- 140°C). A theoretical model is proposed for a quantitative description of the polarized fluorescence emission and its dependence on the squeezing parameter. It is shown that chloroplasts are aligned as disc-shaped particles with no detectable deformation of their ultrastructure. The fluorescence emission and the polarization ratio spectra as well as the calculated orientation angles between the transition dipoles and the membrane plane were similar to those obtained with magnetically oriented chloroplasts.

KW - (Maize)

KW - Chloroplast orientation

KW - Fluorescence

KW - Polarization

KW - Polyacrylamide gel

UR - http://www.scopus.com/inward/record.url?scp=26844432274&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=26844432274&partnerID=8YFLogxK

U2 - 10.1016/0005-2728(83)90128-7

DO - 10.1016/0005-2728(83)90128-7

M3 - Article

AN - SCOPUS:26844432274

VL - 723

SP - 287

EP - 293

JO - Biochimica et Biophysica Acta - Bioenergetics

JF - Biochimica et Biophysica Acta - Bioenergetics

SN - 0005-2728

IS - 2

ER -