Analysis of linearly polarized fluorescence of chloroplasts oriented in polyacrylamide gel

A. O. Ganago, Gy I. Garab, Á Faludi-Dániel

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Linearly polarized fluorescence was measured in mesophyll chloroplasts of maize aligned in polyacrylamide gel. The dependence of fluorescence polarization on the squeezing parameter of the gel sample was studied at room temperature. Polarized fluorescence emission and polarization ratio spectra were recorded at low temperature (- 140°C). A theoretical model is proposed for a quantitative description of the polarized fluorescence emission and its dependence on the squeezing parameter. It is shown that chloroplasts are aligned as disc-shaped particles with no detectable deformation of their ultrastructure. The fluorescence emission and the polarization ratio spectra as well as the calculated orientation angles between the transition dipoles and the membrane plane were similar to those obtained with magnetically oriented chloroplasts.

Original languageEnglish
Pages (from-to)287-293
Number of pages7
JournalBBA - Bioenergetics
Volume723
Issue number2
DOIs
Publication statusPublished - May 27 1983

Keywords

  • (Maize)
  • Chloroplast orientation
  • Fluorescence
  • Polarization
  • Polyacrylamide gel

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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