Analysis of intracellular calcium signaling in human embryonic stem cells

Adrienn Péntek, K. Pászty, A. Apáti

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Measurement of changes in intracellular calcium concentration is one of the most common and useful tools for studying signal transduction pathways or cellular responses in basic research and drug screening purposes as well. Increasing number of such applications using human pluripotent stem cells and their derivatives requires development of calcium signal measurements for this special cell type. Here we describe  a modified protocol for analysis of calcium signaling events in human embryonic stem cells, which can beused for other pluripotent cell types (such as iPSC) or their differentiated offspring as well.

Original languageEnglish
Pages (from-to)141-147
Number of pages7
JournalMethods in molecular biology (Clifton, N.J.)
Volume1307
DOIs
Publication statusPublished - 2015

Fingerprint

Calcium Signaling
Calcium
Pluripotent Stem Cells
Preclinical Drug Evaluations
Signal Transduction
Research
Human Embryonic Stem Cells

Keywords

  • Calcium signaling
  • Confocal microscopy
  • Fl uorescent calcium indicators
  • Genetically encoded calcium indicators
  • Human embryonic stem cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Analysis of intracellular calcium signaling in human embryonic stem cells. / Péntek, Adrienn; Pászty, K.; Apáti, A.

In: Methods in molecular biology (Clifton, N.J.), Vol. 1307, 2015, p. 141-147.

Research output: Contribution to journalArticle

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