Analysis of IMP3 expression in normal and neoplastic human pituitary tissues

Alberto Righi, Shuya Zhang, Long Jin, Bernd W. Scheithauer, Kalman Kovacs, Gabor Kovacs, Miklos I. Goth, Marta Korbonits, Ricardo V. Lloyd

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Insulin-like growth factor II mRNA-binding protein 3 (IMP3) is an oncofetal protein highly expressed in fetal tissue and malignant tumors but rarely found in adult benign tissues. In various tumors, IMP3 expression is correlated with increased tumor aggressiveness and reduced overall survival. To our knowledge, IMP3 expression has not been investigated in pituitary tumors. We analyzed the immunohistochemical expression of IMP3 in five normal pituitary tissues and 75 pituitary tumors (64 adenomas and 11 carcinomas) to determine if specific tumor types expressed IMP3 and if there were differences in IMP3 expression between adenomas and carcinomas. Immunohistochemical analysis showed that IMP3 was positive in four (80%) normal pituitaries with focal stain in a subset of normal anterior pituitary cells. IMP3 was expressed in 31% (20/64) of adenomas and in 36% (4/11) of carcinomas. A slightly higher level of IMP3 expression was observed in PRL-GH-TSH adenomas compared to the other types of pituitary adenomas. Expression of IMP3 was not significantly higher in carcinomas than in adenomas (p∈=∈0.737). RT-PCR and Western Blotting supported the heterogeneous expression of IMP3. These results indicate that IMP3 is expressed both in normal and in neoplastic pituitary gland tissues without significant differences in expression levels in pituitary carcinomas.

Original languageEnglish
Pages (from-to)25-31
Number of pages7
JournalEndocrine Pathology
Volume21
Issue number1
DOIs
Publication statusPublished - Mar 1 2010

Keywords

  • Adenoma
  • Carcinoma
  • IMP3
  • Pituitary

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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  • Cite this

    Righi, A., Zhang, S., Jin, L., Scheithauer, B. W., Kovacs, K., Kovacs, G., Goth, M. I., Korbonits, M., & Lloyd, R. V. (2010). Analysis of IMP3 expression in normal and neoplastic human pituitary tissues. Endocrine Pathology, 21(1), 25-31. https://doi.org/10.1007/s12022-009-9096-9