Analysis of humic substances conformation by X-ray and neutron diffusion scattering methods

A. A. Timchenko, O. E. Trubetskaya, O. A. Trubetskoy, V. Aksenov, M. V. Avdeev, R. Oberthur, H. Kihara, I. N. Serdyuk

Research output: Contribution to journalArticle

Abstract

Samples of humic substances (HS) of black earth and compost and their chromatographic fractions (A, B, C + D) have been obtained by a combination of gel-chromatography and electrophoresis aimed to decrease their dimensions and to increase the electrophoretic mobility and studied by small-angle neutron and synchrotron X-ray scattering methods. It has been showed that the initial HS preparations and their fractions are polydisperse, the low-molecular mass fractions (B, C + D) being more polydisperse than the high-molecular mass fraction (A). It has been found that HS of black earth are enriched with low-molecular mass fractions as compared to the HS of compost. Analysis of particle conformation permitted us to conclude that HS macromolecules in A and B fractions of black earth have a branched coiled structure. At the same time, the conformation of macromolecules in the C + D fraction of black earth and in all compost fractions has a rod-like (elongated) structure. Investigation of the large-angle portion of scattering curves both of initial HS specimens and their fractions has showed that all the studied compounds belong to the class of mass fractals.

Original languageEnglish
Pages (from-to)603-614
Number of pages12
JournalSurface Investigation X-Ray, Synchrotron and Neutron Techniques
Volume15
Issue number4
Publication statusPublished - 2000

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Humic Substances
Conformations
Neutrons
Scattering
neutrons
X rays
Molecular mass
Earth (planet)
scattering
x rays
Macromolecules
macromolecules
Electrophoretic mobility
Chromatography
Electrophoresis
X ray scattering
Synchrotrons
Fractals
gel chromatography
Gels

ASJC Scopus subject areas

  • Surfaces and Interfaces

Cite this

Timchenko, A. A., Trubetskaya, O. E., Trubetskoy, O. A., Aksenov, V., Avdeev, M. V., Oberthur, R., ... Serdyuk, I. N. (2000). Analysis of humic substances conformation by X-ray and neutron diffusion scattering methods. Surface Investigation X-Ray, Synchrotron and Neutron Techniques, 15(4), 603-614.

Analysis of humic substances conformation by X-ray and neutron diffusion scattering methods. / Timchenko, A. A.; Trubetskaya, O. E.; Trubetskoy, O. A.; Aksenov, V.; Avdeev, M. V.; Oberthur, R.; Kihara, H.; Serdyuk, I. N.

In: Surface Investigation X-Ray, Synchrotron and Neutron Techniques, Vol. 15, No. 4, 2000, p. 603-614.

Research output: Contribution to journalArticle

Timchenko, AA, Trubetskaya, OE, Trubetskoy, OA, Aksenov, V, Avdeev, MV, Oberthur, R, Kihara, H & Serdyuk, IN 2000, 'Analysis of humic substances conformation by X-ray and neutron diffusion scattering methods', Surface Investigation X-Ray, Synchrotron and Neutron Techniques, vol. 15, no. 4, pp. 603-614.
Timchenko, A. A. ; Trubetskaya, O. E. ; Trubetskoy, O. A. ; Aksenov, V. ; Avdeev, M. V. ; Oberthur, R. ; Kihara, H. ; Serdyuk, I. N. / Analysis of humic substances conformation by X-ray and neutron diffusion scattering methods. In: Surface Investigation X-Ray, Synchrotron and Neutron Techniques. 2000 ; Vol. 15, No. 4. pp. 603-614.
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AU - Trubetskaya, O. E.

AU - Trubetskoy, O. A.

AU - Aksenov, V.

AU - Avdeev, M. V.

AU - Oberthur, R.

AU - Kihara, H.

AU - Serdyuk, I. N.

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AB - Samples of humic substances (HS) of black earth and compost and their chromatographic fractions (A, B, C + D) have been obtained by a combination of gel-chromatography and electrophoresis aimed to decrease their dimensions and to increase the electrophoretic mobility and studied by small-angle neutron and synchrotron X-ray scattering methods. It has been showed that the initial HS preparations and their fractions are polydisperse, the low-molecular mass fractions (B, C + D) being more polydisperse than the high-molecular mass fraction (A). It has been found that HS of black earth are enriched with low-molecular mass fractions as compared to the HS of compost. Analysis of particle conformation permitted us to conclude that HS macromolecules in A and B fractions of black earth have a branched coiled structure. At the same time, the conformation of macromolecules in the C + D fraction of black earth and in all compost fractions has a rod-like (elongated) structure. Investigation of the large-angle portion of scattering curves both of initial HS specimens and their fractions has showed that all the studied compounds belong to the class of mass fractals.

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