### Abstract

Samples of humic substances (HS) of black earth and compost and their chromatographic fractions (A, B, C + D) have been obtained by a combination of gel-chromatography and electrophoresis aimed to decrease their dimensions and to increase the electrophoretic mobility and studied by small-angle neutron and synchrotron X-ray scattering methods. It has been showed that the initial HS preparations and their fractions are polydisperse, the low-molecular mass fractions (B, C + D) being more polydisperse than the high-molecular mass fraction (A). It has been found that HS of black earth are enriched with low-molecular mass fractions as compared to the HS of compost. Analysis of particle conformation permitted us to conclude that HS macromolecules in A and B fractions of black earth have a branched coiled structure. At the same time, the conformation of macromolecules in the C + D fraction of black earth and in all compost fractions has a rod-like (elongated) structure. Investigation of the large-angle portion of scattering curves both of initial HS specimens and their fractions has showed that all the studied compounds belong to the class of mass fractals.

Original language | English |
---|---|

Pages (from-to) | 603-614 |

Number of pages | 12 |

Journal | Surface Investigation X-Ray, Synchrotron and Neutron Techniques |

Volume | 15 |

Issue number | 4 |

Publication status | Published - 2000 |

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### ASJC Scopus subject areas

- Surfaces and Interfaces

### Cite this

*Surface Investigation X-Ray, Synchrotron and Neutron Techniques*,

*15*(4), 603-614.

**Analysis of humic substances conformation by X-ray and neutron diffusion scattering methods.** / Timchenko, A. A.; Trubetskaya, O. E.; Trubetskoy, O. A.; Aksenov, V.; Avdeev, M. V.; Oberthur, R.; Kihara, H.; Serdyuk, I. N.

Research output: Contribution to journal › Article

*Surface Investigation X-Ray, Synchrotron and Neutron Techniques*, vol. 15, no. 4, pp. 603-614.

}

TY - JOUR

T1 - Analysis of humic substances conformation by X-ray and neutron diffusion scattering methods

AU - Timchenko, A. A.

AU - Trubetskaya, O. E.

AU - Trubetskoy, O. A.

AU - Aksenov, V.

AU - Avdeev, M. V.

AU - Oberthur, R.

AU - Kihara, H.

AU - Serdyuk, I. N.

PY - 2000

Y1 - 2000

N2 - Samples of humic substances (HS) of black earth and compost and their chromatographic fractions (A, B, C + D) have been obtained by a combination of gel-chromatography and electrophoresis aimed to decrease their dimensions and to increase the electrophoretic mobility and studied by small-angle neutron and synchrotron X-ray scattering methods. It has been showed that the initial HS preparations and their fractions are polydisperse, the low-molecular mass fractions (B, C + D) being more polydisperse than the high-molecular mass fraction (A). It has been found that HS of black earth are enriched with low-molecular mass fractions as compared to the HS of compost. Analysis of particle conformation permitted us to conclude that HS macromolecules in A and B fractions of black earth have a branched coiled structure. At the same time, the conformation of macromolecules in the C + D fraction of black earth and in all compost fractions has a rod-like (elongated) structure. Investigation of the large-angle portion of scattering curves both of initial HS specimens and their fractions has showed that all the studied compounds belong to the class of mass fractals.

AB - Samples of humic substances (HS) of black earth and compost and their chromatographic fractions (A, B, C + D) have been obtained by a combination of gel-chromatography and electrophoresis aimed to decrease their dimensions and to increase the electrophoretic mobility and studied by small-angle neutron and synchrotron X-ray scattering methods. It has been showed that the initial HS preparations and their fractions are polydisperse, the low-molecular mass fractions (B, C + D) being more polydisperse than the high-molecular mass fraction (A). It has been found that HS of black earth are enriched with low-molecular mass fractions as compared to the HS of compost. Analysis of particle conformation permitted us to conclude that HS macromolecules in A and B fractions of black earth have a branched coiled structure. At the same time, the conformation of macromolecules in the C + D fraction of black earth and in all compost fractions has a rod-like (elongated) structure. Investigation of the large-angle portion of scattering curves both of initial HS specimens and their fractions has showed that all the studied compounds belong to the class of mass fractals.

UR - http://www.scopus.com/inward/record.url?scp=0033732982&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033732982&partnerID=8YFLogxK

M3 - Article

VL - 15

SP - 603

EP - 614

JO - Journal of Surface Investigation

JF - Journal of Surface Investigation

SN - 1027-4510

IS - 4

ER -