In the present work method has been developed for the closed-vessel microwave digestion of human blood, plasma and hair samples and for the multielemental analysis of the prepared samples using ICP-OES and GAAS methods. The method developed has been successfully applied for the analysis of samples from haemodialysed patients (n=30) and healthy controls (n=14). Significant differences of some studied analytes were observed between the blood (Mg and Fe) and plasma (B, Mg, K, Ca and Fe) samples of the haemodialysed patients and the control group. However, more considerable differences were found between the two groups in the case of hair samples. Our opinion is that after sufficient preliminary studies the trace element analysis of the hair samples from haemodialysed patients may become an important tool for the monitoring of trace element concentrations and to prevent poisoning from accumulated toxic metals. Water samples were taken from different parts of the apparatus used for cleaning of the water applied for haemodialysis. We observed that the aluminium concentration of the tap water used may affect the Al content of the cleaned water. However, due to the successful cleaning procedure, the aluminium content of the cleaned water remained well below the allowed limit. The evaluation of the data obtained is difficult because 1) the reference ranges given in different publications vary considerably, 2) the normal range can be different for the populations living at various locations, and 3) the deviations of the results caused by the biological diversity can be very high. To eliminate these problems and to draw more general conclusions we would like to analyse a huge number of samples both from haemodialysed patients and healthy controls in our future work.
|Translated title of the contribution||Analysis of human blood, plasma and hair samples using ICP-OES, GAAS and spectographic methods|
|Number of pages||8|
|Journal||Magyar Kemiai Folyoirat, Kemiai Kozlemenyek|
|Publication status||Published - May 1 2000|
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