Analysis of gene expression profiles in melanoma cells with acquired resistance against antineoplastic drugs

B. Györffy, Violeta Serra, Verena Materna, Reinhold Schäfer, Manfred Dietel, Dirk Schadendorf, Hermann Lage

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Resistance to various antineoplastic agents is common in the clinical management of malignant melanoma. The biological mechanisms conferring these different drug-resistant phenotypes are still unclear. To identify potential factors mediating drug resistance to melanoma cells, the mRNA expression profiles of the parental drug-sensitive human melanoma cell line MeWo and four derived drug-resistant sublines with acquired resistance against four commonly used drugs for melanoma treatment (cisplatin, etoposide, fotemustine and vindesine) were analysed. We investigated cDNA arrays with 43 000 cDNA clones (∼30 000 unique genes) to study the expression patterns of these cell lines. We were able to simultaneously extract new candidate genes associated with drug resistance in malignant melanoma and to correlate the present findings with previously described resistance-associated genes. Using hierarchical clustering and analysing the overlap of genes with altered expression, we detected similarities between the expression signatures related to cisplatin and fotemustine resistance. The resistance against vindesine required a minimal set of changes in gene expression relative to the parental MeWo cell line. Our study provides new data that may be used to obtain further insight into the resistance characteristics of malignant melanoma.

Original languageEnglish
Pages (from-to)147-155
Number of pages9
JournalMelanoma Research
Volume16
Issue number2
DOIs
Publication statusPublished - Apr 2006

Fingerprint

Neoplasm Drug Resistance
Transcriptome
Melanoma
fotemustine
Vindesine
Drug Resistance
Cell Line
Pharmaceutical Preparations
Cisplatin
Genes
Etoposide
Oligonucleotide Array Sequence Analysis
Antineoplastic Agents
Cluster Analysis
Complementary DNA
Clone Cells
Phenotype
Gene Expression
Messenger RNA

Keywords

  • Drug resistance
  • Melanoma
  • MeWo

ASJC Scopus subject areas

  • Cancer Research
  • Dermatology

Cite this

Analysis of gene expression profiles in melanoma cells with acquired resistance against antineoplastic drugs. / Györffy, B.; Serra, Violeta; Materna, Verena; Schäfer, Reinhold; Dietel, Manfred; Schadendorf, Dirk; Lage, Hermann.

In: Melanoma Research, Vol. 16, No. 2, 04.2006, p. 147-155.

Research output: Contribution to journalArticle

Györffy, B. ; Serra, Violeta ; Materna, Verena ; Schäfer, Reinhold ; Dietel, Manfred ; Schadendorf, Dirk ; Lage, Hermann. / Analysis of gene expression profiles in melanoma cells with acquired resistance against antineoplastic drugs. In: Melanoma Research. 2006 ; Vol. 16, No. 2. pp. 147-155.
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