Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis

G. Vasas, A. Gáspár, Csilla Páger, Gyula Surányi, Csaba Máthé, Márta M. Hamvas, G. Borbély

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) were applied to the simultaneous separation of cyanobacterial toxins (anatoxin-a, microcystin-LR, cylindrospermopsin). The analytical performance data of both methods, optimized for the three toxins, were similar with a precision of migration times smaller than 0.8 RSD% and a detection limit in the range of 1-4 μg/mL, using spectrophotometric detection at 230 nm. Both methods were applied to an analysis of cyanotoxins in water bloom samples and crude cyanobacterial extracts. The results obtained indicate that, for complex matrices, the sequential application of CZE and MEKC is necessary. It is recommended to use both CE techniques for the analysis of the same sample in order to confirm the results by an orthogonal approach.

Original languageEnglish
Pages (from-to)108-115
Number of pages8
JournalElectrophoresis
Volume25
Issue number1
DOIs
Publication statusPublished - Jan 2004

Fingerprint

Capillary electrophoresis
Capillary Electrophoresis
Chromatography
Electrophoresis
Complex Mixtures
Limit of Detection
Water
cylindrospermopsin
cyanoginosin LR
cyanobacterial toxin
anatoxin a

Keywords

  • Anatoxin-a
  • Capillary electrophoresis
  • Cyanobacterial toxins
  • Cylindrospermopsin
  • Microcystin-LR

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis. / Vasas, G.; Gáspár, A.; Páger, Csilla; Surányi, Gyula; Máthé, Csaba; Hamvas, Márta M.; Borbély, G.

In: Electrophoresis, Vol. 25, No. 1, 01.2004, p. 108-115.

Research output: Contribution to journalArticle

Vasas, G. ; Gáspár, A. ; Páger, Csilla ; Surányi, Gyula ; Máthé, Csaba ; Hamvas, Márta M. ; Borbély, G. / Analysis of cyanobacterial toxins (anatoxin-a, cylindrospermopsin, microcystin-LR) by capillary electrophoresis. In: Electrophoresis. 2004 ; Vol. 25, No. 1. pp. 108-115.
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