Natural products are among the largest and most diverse groups of plant secondary metabolites. Advances in modern genetic technology have enabled scientists to engineer biosynthetic pathways with the promise of generating novel chemical entities. In this study, the authors used an extensive database of secondary metabolite-related enzymes for cloning and testing Catharanthus roseus and other plant genes of interest. They discuss the use of accelerated solvent extraction, high performance liquid chromatography and capillary electrophoresis (CE) to monitor changes in combinatorial genetics-mediated secondary metabolite generation. Because of the lower sample requirement and higher sensitivity, the authors found CE to be their preferred separation method in this study.
|Number of pages||8|
|Publication status||Published - Feb 1 2004|
ASJC Scopus subject areas
- Analytical Chemistry