Analysis of chloroplast and mitochondrial DNAs in asymmetric somatic hybrids between tobacco and carrot

M. A. Smith, A. Pay, D. Dudits

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Chloroplast and mitochondrial DNAs have been examined by comparison of restriction enzyme patterns in asymmetric hybrid plants, resulting from the fusion between leaf mesophyll protoplasts of Nicotiana tabacum (Solanaceae), and irradiated cell culture protoplasts of Daucus carota (Umbellifereae). These somatic hybrids with normal tobacco morphology were selected as a consequence of the transfer of methotrexate and 5-methyltryptophan resistance from carrot to tobacco. The restriction patterns of chloroplast DNAs in somatic hybrids were indistinguishable from the tobacco parent. However, we found somatic hybrids with mitochondrial DNA significantly different from either parent, as judged by analysis of fragment distribution after restriction enzyme digestion. The possible formation of altered mitochondrial DNA molecules as the result of parasexual hybrid production between two phylogenetically highly divergent plant species will be discussed.

Original languageEnglish
Pages (from-to)641-644
Number of pages4
JournalTheoretical and Applied Genetics
Volume77
Issue number5
DOIs
Publication statusPublished - May 1 1989

Keywords

  • Carrot
  • Chloroplast DNA
  • Mitochondrial DNA
  • Somatic hybridization
  • Tobacco

ASJC Scopus subject areas

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

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