Analysis of apoptosis in isolated primary cardiac myocytes.

Adel Mandl, A. Tóth, Peter Erhardt

Research output: Contribution to journalArticle

Abstract

Acute myocardial infarction represents the leading cause of morbidity and mortality in the western societies. Importantly, both apoptosis and necrosis of cardiomyocytes have been implicated in the pathomechanism of myocardial infarction. The simplest way to analyze apoptosis in cardiac cells is the application of isolated neonatal primary cardiac myocytes, in which ischemia/reperfusion can be mimicked in vitro by exposing them to hypoxia and serum starvation, followed by restored oxygen and serum conditions, referred to as hypoxia/reoxygenation. In this chapter, we describe protocols routinely applied in our lab for investigating cardiomyocyte apoptosis. In summary, a better understanding of the apoptotic pathways and their regulation in the heart will potentially yield novel therapeutic targets for cardiac infarction.

Original languageEnglish
Pages (from-to)293-311
Number of pages19
JournalMethods in molecular biology (Clifton, N.J.)
Volume559
DOIs
Publication statusPublished - 2009

Fingerprint

Cardiac Myocytes
Apoptosis
Myocardial Infarction
Starvation
Serum
Infarction
Reperfusion
Necrosis
Ischemia
Oxygen
Morbidity
Mortality
Hypoxia
Therapeutics

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Analysis of apoptosis in isolated primary cardiac myocytes. / Mandl, Adel; Tóth, A.; Erhardt, Peter.

In: Methods in molecular biology (Clifton, N.J.), Vol. 559, 2009, p. 293-311.

Research output: Contribution to journalArticle

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