Analysis of amino acids and biogenic amines in biological tissues as their o-phthalaldehyde/ethanethiol/fluorenylmethyl chloroformate derivatives by high-performance liquid chromatography. A deproteinization study

Á Korös, R. Hanczkó, A. Jámbor, Y. Qian, A. Perl, I. Molnár-Perl

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27 Citations (Scopus)

Abstract

The extraction of ornithine, lysine, putrescine, cadaverine, 1,7-diaminoheptane, spermidine and spermine from biological tissues was optimized for HPLC quantitation as their o-phthalaldehyde/ethanethiol/fluorenylmethyl chloroformate (OPA/ET/FMOC) derivatives. In applying perchloric acid deproteinization two approaches have been followed: (i) deproteinization with subsequent neutralization by potassium hydroxide and lyophilization, and (ii) deproteinization without neutralization and lyophilization. Neutralization and lyophilization resulted in the loss of free biogenic amines. HPLC analysis of ornithine (Orn), lysine (Lys), putrescine (Put), cadaverine (Cad), 1,7-diaminoheptane (Dah), spermidine (Spd) and spermine (Spm) content of biological tissues as their OPA/ET/FMOC derivatives was performed in the supernatant of perchloric acid-deproteinized samples (model solutions and tissues) with an average reproducibility of ≤2.6% relative standard deviation (RSD), including recovery of sample treatment and chromatography.

Original languageEnglish
Pages (from-to)46-55
Number of pages10
JournalJournal of Chromatography A
Volume1149
Issue number1
DOIs
Publication statusPublished - May 11 2007

Fingerprint

o-Phthalaldehyde
Biogenic Amines
Freeze Drying
High performance liquid chromatography
Cadaverine
Ornithine
Putrescine
Spermidine
Spermine
High Pressure Liquid Chromatography
Tissue
Derivatives
Amino Acids
Lysine
Chromatography
Recovery
ethanethiol
1,7-diaminoheptane
Perchloric Acid
Therapeutics

Keywords

  • Amino acids
  • Biogenic amines
  • Biological tissues
  • HPLC-UV(DAD)/fluorescence detection
  • OPA/ET/FMOC derivatization
  • Perchloric acid deproteinization
  • Sample preparation

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

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title = "Analysis of amino acids and biogenic amines in biological tissues as their o-phthalaldehyde/ethanethiol/fluorenylmethyl chloroformate derivatives by high-performance liquid chromatography. A deproteinization study",
abstract = "The extraction of ornithine, lysine, putrescine, cadaverine, 1,7-diaminoheptane, spermidine and spermine from biological tissues was optimized for HPLC quantitation as their o-phthalaldehyde/ethanethiol/fluorenylmethyl chloroformate (OPA/ET/FMOC) derivatives. In applying perchloric acid deproteinization two approaches have been followed: (i) deproteinization with subsequent neutralization by potassium hydroxide and lyophilization, and (ii) deproteinization without neutralization and lyophilization. Neutralization and lyophilization resulted in the loss of free biogenic amines. HPLC analysis of ornithine (Orn), lysine (Lys), putrescine (Put), cadaverine (Cad), 1,7-diaminoheptane (Dah), spermidine (Spd) and spermine (Spm) content of biological tissues as their OPA/ET/FMOC derivatives was performed in the supernatant of perchloric acid-deproteinized samples (model solutions and tissues) with an average reproducibility of ≤2.6{\%} relative standard deviation (RSD), including recovery of sample treatment and chromatography.",
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T1 - Analysis of amino acids and biogenic amines in biological tissues as their o-phthalaldehyde/ethanethiol/fluorenylmethyl chloroformate derivatives by high-performance liquid chromatography. A deproteinization study

AU - Korös, Á

AU - Hanczkó, R.

AU - Jámbor, A.

AU - Qian, Y.

AU - Perl, A.

AU - Molnár-Perl, I.

PY - 2007/5/11

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AB - The extraction of ornithine, lysine, putrescine, cadaverine, 1,7-diaminoheptane, spermidine and spermine from biological tissues was optimized for HPLC quantitation as their o-phthalaldehyde/ethanethiol/fluorenylmethyl chloroformate (OPA/ET/FMOC) derivatives. In applying perchloric acid deproteinization two approaches have been followed: (i) deproteinization with subsequent neutralization by potassium hydroxide and lyophilization, and (ii) deproteinization without neutralization and lyophilization. Neutralization and lyophilization resulted in the loss of free biogenic amines. HPLC analysis of ornithine (Orn), lysine (Lys), putrescine (Put), cadaverine (Cad), 1,7-diaminoheptane (Dah), spermidine (Spd) and spermine (Spm) content of biological tissues as their OPA/ET/FMOC derivatives was performed in the supernatant of perchloric acid-deproteinized samples (model solutions and tissues) with an average reproducibility of ≤2.6% relative standard deviation (RSD), including recovery of sample treatment and chromatography.

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