Analysis of ameloblastomas by comparative genomic hybridization and fluorescence in situ hybridization

Makoto Toida, M. Balázs, Andrea Treszl, Z. Rákosy, Keizo Kato, Yutaka Yamazaki, Toshiaki Matsui, Tatsuhiko Suwa, Daijiro Hatakeyama, Hiroki Makita, Sojiro Mori, Tomomi Yamashita, Toshiyuki Shibata, R. Ádány

Research output: Contribution to journalArticle

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Abstract

In order to characterize the chromosomal alterations in ameloblastomas, a combination of comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH) techniques was performed on 9 tumors. Chromosomal alterations including a gain at 1q and losses at 1pter, 10q, and 22q could be detected by CGH only in 1 tumor. Interphase FISH analysis, using centromeric probes for chromosomes 1, 10, and 22 as well as region-specific probes for 1p36 and 10q26, revealed the most frequent alterations to exist in the tumor with the abnormal CGH profile. These alterations included marked to slight increases of monosomic cells for chromosome 10 (91.5%), 10q26 (35.8%), 1p36 (24.4%), and chromosome 22 (18.8%), as well as significant elevations of trisomic cells for chromosome 1 (41.2%). Moreover, FISH analysis revealed a frequent loss of chromosome 22 in all tumors examined, except for one lesion, indicating that loss of the entire or a part of this chromosome is a common event in ameloblastomas, possibly being a predisposing factor to ameloblastoma tumorigenesis.

Original languageEnglish
Pages (from-to)99-104
Number of pages6
JournalCancer Genetics and Cytogenetics
Volume159
Issue number2
DOIs
Publication statusPublished - Jun 2005

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Ameloblastoma
Comparative Genomic Hybridization
Fluorescence In Situ Hybridization
Chromosomes, Human, Pair 22
In Situ Hybridization
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 1
Neoplasms
Interphase
Causality
Carcinogenesis
Chromosomes

ASJC Scopus subject areas

  • Cancer Research
  • Genetics
  • Molecular Biology

Cite this

Analysis of ameloblastomas by comparative genomic hybridization and fluorescence in situ hybridization. / Toida, Makoto; Balázs, M.; Treszl, Andrea; Rákosy, Z.; Kato, Keizo; Yamazaki, Yutaka; Matsui, Toshiaki; Suwa, Tatsuhiko; Hatakeyama, Daijiro; Makita, Hiroki; Mori, Sojiro; Yamashita, Tomomi; Shibata, Toshiyuki; Ádány, R.

In: Cancer Genetics and Cytogenetics, Vol. 159, No. 2, 06.2005, p. 99-104.

Research output: Contribution to journalArticle

Toida, M, Balázs, M, Treszl, A, Rákosy, Z, Kato, K, Yamazaki, Y, Matsui, T, Suwa, T, Hatakeyama, D, Makita, H, Mori, S, Yamashita, T, Shibata, T & Ádány, R 2005, 'Analysis of ameloblastomas by comparative genomic hybridization and fluorescence in situ hybridization', Cancer Genetics and Cytogenetics, vol. 159, no. 2, pp. 99-104. https://doi.org/10.1016/j.cancergencyto.2004.10.004
Toida, Makoto ; Balázs, M. ; Treszl, Andrea ; Rákosy, Z. ; Kato, Keizo ; Yamazaki, Yutaka ; Matsui, Toshiaki ; Suwa, Tatsuhiko ; Hatakeyama, Daijiro ; Makita, Hiroki ; Mori, Sojiro ; Yamashita, Tomomi ; Shibata, Toshiyuki ; Ádány, R. / Analysis of ameloblastomas by comparative genomic hybridization and fluorescence in situ hybridization. In: Cancer Genetics and Cytogenetics. 2005 ; Vol. 159, No. 2. pp. 99-104.
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