Analysis of 5′-ends of short DNA fragments excreted by phytohemagglutinin stimulated lymphocytes

Gaspar Banfalvi, Krzysztof Szyfter, Ferenc Antoni

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Human peripheral blood lymphocytes were stimulated with phytohemagglutinin and the excreted DNA was isolated from the medium after four days of incubation of cells. The excreted DNA was labeled at the 5′-end with [γ-32P]ATP and polynucleotide kinase. Analysis of the end-labeled material revealed a size distribution with a chain length of 6 - 60 nucleotides. These short DNA fragments did not contain ribonucleotides at their 5′-termini. P1 nuclease digestion did not release specific deoxyribonucleoside monophosphates from the 5′-end of the excreted DNA fragments. These results point to the non-specific degradation of DNA excreted by stimulated lymphocytes.

Original languageEnglish
Pages (from-to)140-146
Number of pages7
JournalBiochemical and biophysical research communications
Volume118
Issue number1
DOIs
Publication statusPublished - Jan 13 1984

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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