Analysis of 1-Aminopyrene-3,6,8-trisulfonate-Derivatized Oligosaccharides by Capillary Electrophoresis with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Hirofumi Suzuki, Odilo Muller, A. Guttman, Barry L. Karger

Research output: Contribution to journalArticle

88 Citations (Scopus)

Abstract

Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), has been used for structure characterization of 1-aminopyrene-3,6,8-trisulfonate (APTS)-derivatized oligosaccharides previously separated by capillary electrophoresis (CE). The resolved components were first isolated by employing an automated high-resolution fraction collector. Using on-probe sample cleanup with a cation-exchange resin and a matrix mixture of (1:1) 6-hydroxypicolinic acid and 3-hydroxypicolinic acid, APTS-labeled oligosaccharides were successfully detected by MALDI-TOF MS in the negative ionization mode. Each APTS-labeled oligosaccharide produced single [M - H]-1 peaks. Detection limits for standard APTS-derivatized oligosaccharide (maltoheptaose) were down to 30 fmol. APTS labeled maltooligosaccharides and various standard carbohydrates were separated and collected by CE, followed by molecular weight determination with MALDI-TOF MS. The mass spectral characterization enhances the power of the CE analysis of oligosaccharides.

Original languageEnglish
Pages (from-to)4554-4559
Number of pages6
JournalAnalytical Chemistry
Volume69
Issue number22
Publication statusPublished - Nov 15 1997

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Capillary electrophoresis
Oligosaccharides
Ionization
Mass spectrometry
Desorption
Lasers
Cation Exchange Resins
Laser modes
Molecular weight
Carbohydrates
1-aminopyrene

ASJC Scopus subject areas

  • Analytical Chemistry

Cite this

Analysis of 1-Aminopyrene-3,6,8-trisulfonate-Derivatized Oligosaccharides by Capillary Electrophoresis with Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. / Suzuki, Hirofumi; Muller, Odilo; Guttman, A.; Karger, Barry L.

In: Analytical Chemistry, Vol. 69, No. 22, 15.11.1997, p. 4554-4559.

Research output: Contribution to journalArticle

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