Analysis-based parameter estimation of an in vitro transcription-translation system

Zoltan A. Tuza, Dan Siegal-Gaskins, Jongmin Kim, G. Szederkényi

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Recent advances in measurement technology provide us with rich source of data for estimating parameters in biomolecular circuit models, particularly in simplified in vitro transcription-translation systems, so-called molecular 'bread-boards'. In this paper, we elaborate on a mass action type dynamic model for such an in vitro system and detail a parameter estimation procedure that may be used with time series data containing information about both transcriptional and translational stages of gene expression. The identification process is supported by structural identifiability analysis to ensure proper model structure. Statistical analysis and validation of the estimated parameter set help us to understand the characteristics of point estimation results.

Original languageEnglish
Title of host publication2015 European Control Conference, ECC 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages1560-1566
Number of pages7
ISBN (Print)9783952426937
DOIs
Publication statusPublished - Nov 16 2015
EventEuropean Control Conference, ECC 2015 - Linz, Austria
Duration: Jul 15 2015Jul 17 2015

Other

OtherEuropean Control Conference, ECC 2015
CountryAustria
CityLinz
Period7/15/157/17/15

    Fingerprint

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Tuza, Z. A., Siegal-Gaskins, D., Kim, J., & Szederkényi, G. (2015). Analysis-based parameter estimation of an in vitro transcription-translation system. In 2015 European Control Conference, ECC 2015 (pp. 1560-1566). [7330760] Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/ECC.2015.7330760