Analysis and characterization of heparin impurities

S. Béni, John F K Limtiaco, Cynthia K. Larive

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

This review discusses recent developments in analytical methods available for the sensitive separation, detection and structural characterization of heparin contaminants. The adulteration of raw heparin with oversulfated chondroitin sulfate (OSCS) in 2007-2008 spawned a global crisis resulting in extensive revisions to the pharmacopeia monographs on heparin and prompting the FDA to recommend the development of additional physicochemical methods for the analysis of heparin purity. The analytical chemistry community quickly responded to this challenge, developing a wide variety of innovative approaches, several of which are reported in this special issue. This review provides an overview of methods of heparin isolation and digestion, discusses known heparin contaminants, including OSCS, and summarizes recent publications on heparin impurity analysis using sensors, near-IR, Raman, and NMR spectroscopy, as well as electrophoretic and chromatographic separations.

Original languageEnglish
Pages (from-to)527-539
Number of pages13
JournalAnalytical and Bioanalytical Chemistry
Volume399
Issue number2
DOIs
Publication statusPublished - Jan 2011

Fingerprint

Heparin
Impurities
Chondroitin Sulfates
Pharmacopoeias
Raman Spectrum Analysis
Nuclear magnetic resonance spectroscopy
Raman spectroscopy
Digestion
Infrared spectroscopy
Magnetic Resonance Spectroscopy
Sensors
Chemical analysis

Keywords

  • Adulteration
  • Contamination
  • Heparin
  • Impurity
  • NMR
  • OSCS

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Analysis and characterization of heparin impurities. / Béni, S.; Limtiaco, John F K; Larive, Cynthia K.

In: Analytical and Bioanalytical Chemistry, Vol. 399, No. 2, 01.2011, p. 527-539.

Research output: Contribution to journalArticle

Béni, S. ; Limtiaco, John F K ; Larive, Cynthia K. / Analysis and characterization of heparin impurities. In: Analytical and Bioanalytical Chemistry. 2011 ; Vol. 399, No. 2. pp. 527-539.
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