Anaerobacillus alkaliphilus sp. nov., a novel alkaliphilic and moderately halophilic bacterium

A. Borsodi, Júlia M. Aszalós, Péter Bihari, István Nagy, Peter Schumann, Cathrin Spröer, A. Kovács, K. Bóka, Péter Dobosy, Mihály Óvári, Tibor Szili-Kovács, E. Tóth

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Two alkaliphilic and moderately halophilic bacterial strains B16-10T and Z23-18 characterized by optimal growth at pH 9.0-10.0 and 5 % (w/v) NaCl, were isolated from the rhizosphere soil of the bayonet grass (Bolboschoenus maritimus) in the Kiskunság National Park, Hungary. Cells of both strains stained Gram-positive, were motile straight rods, and formed terminal, ellipsoidal endospores with swollen sporangia. The isolates were facultative anaerobic, catalase positive, oxidase negative. Both strains contained meso-diaminopimelic acid as diagnostic diaminoacid of the peptidoglycan. Menaquinone-7 (MK-7) was the predominant isoprenoid quinone. Anteiso-C15 : 0, C16 : 1ω11c and iso-C14 : 0 were the major cellular fatty acids. The DNA G+C content of both strains was 35.8 mol%. The 16S rRNA gene based phylogenetic analysis revealed that the facultative anaerobic strains B16-10T and Z23-18 showed the highest similarities to the type strains of anaerobic Anaerobacillus isosaccharinicus NB2006T (98.7 and 99.1 %), A. macyae JMM-4T (98.2 and 98.4 %), A. alkalidiazotrophicus MS 6T (97.7 and 98.4 %), A. alkalilacustris Z-0521T (97.5 and 98.3 %) and A. arseniciselenatis DSM 15340T (97.5 and 98.2 %). However, the distinctive phenotypic and genetic results of this study confirmed that strains B16-10T and Z23-18 represent a novel species, for which the name Anaerobacillus alkaliphilus sp. nov. is proposed. The type strain is B16-10T (=DSM 29790T=NCAIM B 02608T).

Original languageEnglish
Pages (from-to)631-637
Number of pages7
JournalInternational journal of systematic and evolutionary microbiology
Volume69
Issue number3
DOIs
Publication statusPublished - Mar 1 2019

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Alkaliphilus
Sporangia
Diaminopimelic Acid
Rhizosphere
Peptidoglycan
Hungary
isoprenoid
Terpenes
Poaceae
rRNA Genes
Catalase
Names
rhizosphere
Oxidoreductases
national park
Fatty Acids
fatty acid
Soil
grass
Bacteria

Keywords

  • alkaliphilic and moderately halophilic bacterium
  • Anaerobacillus
  • soda soil

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Anaerobacillus alkaliphilus sp. nov., a novel alkaliphilic and moderately halophilic bacterium. / Borsodi, A.; Aszalós, Júlia M.; Bihari, Péter; Nagy, István; Schumann, Peter; Spröer, Cathrin; Kovács, A.; Bóka, K.; Dobosy, Péter; Óvári, Mihály; Szili-Kovács, Tibor; Tóth, E.

In: International journal of systematic and evolutionary microbiology, Vol. 69, No. 3, 01.03.2019, p. 631-637.

Research output: Contribution to journalArticle

Borsodi, A. ; Aszalós, Júlia M. ; Bihari, Péter ; Nagy, István ; Schumann, Peter ; Spröer, Cathrin ; Kovács, A. ; Bóka, K. ; Dobosy, Péter ; Óvári, Mihály ; Szili-Kovács, Tibor ; Tóth, E. / Anaerobacillus alkaliphilus sp. nov., a novel alkaliphilic and moderately halophilic bacterium. In: International journal of systematic and evolutionary microbiology. 2019 ; Vol. 69, No. 3. pp. 631-637.
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AU - Nagy, István

AU - Schumann, Peter

AU - Spröer, Cathrin

AU - Kovács, A.

AU - Bóka, K.

AU - Dobosy, Péter

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