An L-arginine-derived factor mediates endotoxin-induced vascular hyposensitivity to calcium

Gillian A. Gray, Géraldine Julou-Schaeffer, Kleber Oury, Ingrid Fleming, J. Parratt, Jean Claude Stoclet

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Aortas removed from rats treated with bacterial endotoxin displayed a reduced sensitivity to calcium (CaCI2, 10 μM-10 mM) in depolarizing medium (100 mM K+). Sensitivity was reduced further in the presence of L-arginine (1 mM) but restored to control by Nω-nitroarginine methyl ester (L-NAME, 300 μM) or NG-monomethyl-L-arginine (L-NMMA, 300 μm), inhibitors of nitric oxide synthesis from L-arginine. Furthermore, addition of methylene blue (10 μm), an inhibitor of soluble guanylate cyclase, restored the contractile response to 10 mM CaCl2. The results suggest that vascular hyposensitivity to calcium involves stimulation of guanylate cyclase subsequent to activation of the L-arginine pathway by endotoxin.

Original languageEnglish
Pages (from-to)89-92
Number of pages4
JournalEuropean Journal of Pharmacology
Volume191
Issue number1
DOIs
Publication statusPublished - Nov 20 1990

Fingerprint

Endotoxins
Blood Vessels
Arginine
omega-N-Methylarginine
Calcium
Guanylate Cyclase
Nitroarginine
Methylene Blue
NG-Nitroarginine Methyl Ester
Aorta
Nitric Oxide
Esters
Soluble Guanylyl Cyclase

Keywords

  • Ca
  • Endotoxin
  • L-Arginine pathway
  • Smooth muscle (vascular)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

An L-arginine-derived factor mediates endotoxin-induced vascular hyposensitivity to calcium. / Gray, Gillian A.; Julou-Schaeffer, Géraldine; Oury, Kleber; Fleming, Ingrid; Parratt, J.; Stoclet, Jean Claude.

In: European Journal of Pharmacology, Vol. 191, No. 1, 20.11.1990, p. 89-92.

Research output: Contribution to journalArticle

Gray, Gillian A. ; Julou-Schaeffer, Géraldine ; Oury, Kleber ; Fleming, Ingrid ; Parratt, J. ; Stoclet, Jean Claude. / An L-arginine-derived factor mediates endotoxin-induced vascular hyposensitivity to calcium. In: European Journal of Pharmacology. 1990 ; Vol. 191, No. 1. pp. 89-92.
@article{d0e500b9b1e845babf5627e110721393,
title = "An L-arginine-derived factor mediates endotoxin-induced vascular hyposensitivity to calcium",
abstract = "Aortas removed from rats treated with bacterial endotoxin displayed a reduced sensitivity to calcium (CaCI2, 10 μM-10 mM) in depolarizing medium (100 mM K+). Sensitivity was reduced further in the presence of L-arginine (1 mM) but restored to control by Nω-nitroarginine methyl ester (L-NAME, 300 μM) or NG-monomethyl-L-arginine (L-NMMA, 300 μm), inhibitors of nitric oxide synthesis from L-arginine. Furthermore, addition of methylene blue (10 μm), an inhibitor of soluble guanylate cyclase, restored the contractile response to 10 mM CaCl2. The results suggest that vascular hyposensitivity to calcium involves stimulation of guanylate cyclase subsequent to activation of the L-arginine pathway by endotoxin.",
keywords = "Ca, Endotoxin, L-Arginine pathway, Smooth muscle (vascular)",
author = "Gray, {Gillian A.} and G{\'e}raldine Julou-Schaeffer and Kleber Oury and Ingrid Fleming and J. Parratt and Stoclet, {Jean Claude}",
year = "1990",
month = "11",
day = "20",
doi = "10.1016/0014-2999(90)94099-J",
language = "English",
volume = "191",
pages = "89--92",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - An L-arginine-derived factor mediates endotoxin-induced vascular hyposensitivity to calcium

AU - Gray, Gillian A.

AU - Julou-Schaeffer, Géraldine

AU - Oury, Kleber

AU - Fleming, Ingrid

AU - Parratt, J.

AU - Stoclet, Jean Claude

PY - 1990/11/20

Y1 - 1990/11/20

N2 - Aortas removed from rats treated with bacterial endotoxin displayed a reduced sensitivity to calcium (CaCI2, 10 μM-10 mM) in depolarizing medium (100 mM K+). Sensitivity was reduced further in the presence of L-arginine (1 mM) but restored to control by Nω-nitroarginine methyl ester (L-NAME, 300 μM) or NG-monomethyl-L-arginine (L-NMMA, 300 μm), inhibitors of nitric oxide synthesis from L-arginine. Furthermore, addition of methylene blue (10 μm), an inhibitor of soluble guanylate cyclase, restored the contractile response to 10 mM CaCl2. The results suggest that vascular hyposensitivity to calcium involves stimulation of guanylate cyclase subsequent to activation of the L-arginine pathway by endotoxin.

AB - Aortas removed from rats treated with bacterial endotoxin displayed a reduced sensitivity to calcium (CaCI2, 10 μM-10 mM) in depolarizing medium (100 mM K+). Sensitivity was reduced further in the presence of L-arginine (1 mM) but restored to control by Nω-nitroarginine methyl ester (L-NAME, 300 μM) or NG-monomethyl-L-arginine (L-NMMA, 300 μm), inhibitors of nitric oxide synthesis from L-arginine. Furthermore, addition of methylene blue (10 μm), an inhibitor of soluble guanylate cyclase, restored the contractile response to 10 mM CaCl2. The results suggest that vascular hyposensitivity to calcium involves stimulation of guanylate cyclase subsequent to activation of the L-arginine pathway by endotoxin.

KW - Ca

KW - Endotoxin

KW - L-Arginine pathway

KW - Smooth muscle (vascular)

UR - http://www.scopus.com/inward/record.url?scp=0025115930&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025115930&partnerID=8YFLogxK

U2 - 10.1016/0014-2999(90)94099-J

DO - 10.1016/0014-2999(90)94099-J

M3 - Article

C2 - 2093001

AN - SCOPUS:0025115930

VL - 191

SP - 89

EP - 92

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1

ER -