An iron-containing superoxide dismutase from Anacystis nidulans

Csaba Cséke, László Horváth, Pál Simon, G. Borbély, Lajos Keszthelyi, Gábor Farkas

Research output: Contribution to journalArticle

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Abstract

Superoxide dismutase (SOD) was isolated and purified from Anacystis nidulans to near electrophoretic homogeneity. The enzyme has a molecular weight of 37,500, as determined by gel filtration and SDS-gel electrophoresis. The enzyme molecule consists of two subunits of identical molecular weight. Proton-induced X-ray elemental analysis (PIXE) showed that the SOD of A. nidulans is an iron-containing enzyme; the Fe:enzyme mol ratio was found to be 1. The EPR spectra indicated that the active center contains high-spin ferric ion. Based on quantitative EPR data, we conclude that essentially all iron ions were detected in the EPR experiments and were present in the Fe3+ high-spin form. Since the Fe:enzyme mol ratio was 1, we suggest that each enzyme contains a 1-Fe3+ active center.Effective g'-values were calculated from computer-simulated spectra and analysis of the g'-value anisotropy of the ±3/2 Kramers doublet made the calculation of crystal field parameters possible. The symmetry of the Fe3+ ion in the SOD molecule was found to be close to rhombic (E/D=0.240)

Original languageEnglish
Pages (from-to)1397-1404
Number of pages8
JournalJournal of Biochemistry
Volume85
Issue number6
Publication statusPublished - Jun 1979

Fingerprint

Superoxide Dismutase
Iron
Enzymes
Paramagnetic resonance
Ions
Gels
Molecular Weight
Molecular weight
Molecules
Anisotropy
Crystal symmetry
Electrophoresis
Homogeneity
Filtration
Gel Chromatography
Protons
Spectrum Analysis
Crystal
X-Rays
Symmetry

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Physiology (medical)
  • Radiology Nuclear Medicine and imaging
  • Molecular Biology
  • Biochemistry

Cite this

Cséke, C., Horváth, L., Simon, P., Borbély, G., Keszthelyi, L., & Farkas, G. (1979). An iron-containing superoxide dismutase from Anacystis nidulans. Journal of Biochemistry, 85(6), 1397-1404.

An iron-containing superoxide dismutase from Anacystis nidulans. / Cséke, Csaba; Horváth, László; Simon, Pál; Borbély, G.; Keszthelyi, Lajos; Farkas, Gábor.

In: Journal of Biochemistry, Vol. 85, No. 6, 06.1979, p. 1397-1404.

Research output: Contribution to journalArticle

Cséke, C, Horváth, L, Simon, P, Borbély, G, Keszthelyi, L & Farkas, G 1979, 'An iron-containing superoxide dismutase from Anacystis nidulans', Journal of Biochemistry, vol. 85, no. 6, pp. 1397-1404.
Cséke C, Horváth L, Simon P, Borbély G, Keszthelyi L, Farkas G. An iron-containing superoxide dismutase from Anacystis nidulans. Journal of Biochemistry. 1979 Jun;85(6):1397-1404.
Cséke, Csaba ; Horváth, László ; Simon, Pál ; Borbély, G. ; Keszthelyi, Lajos ; Farkas, Gábor. / An iron-containing superoxide dismutase from Anacystis nidulans. In: Journal of Biochemistry. 1979 ; Vol. 85, No. 6. pp. 1397-1404.
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