An inter-laboratory evaluation of selective media for the detection and enumeration of yeasts from blue-veined cheese

B. C. Viljoen, A. Knox, L. R. Beuchat, T. Deák, M. Malfeito-Ferreira, T. K. Hansen, A. Hugo, M. Jakobsen, V. Loureiro, A. Lourens-Hattingh, R. Vasdinnyei

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Five countries representative of laboratories 1-5 evaluated 11 different selective media, designed to suppress mould and bacterial growth and support yeasts growth, for the recovery of yeast populations from blue veined cheeses. In addition, qualitative results were also incorporated. The yeast enumeration values were subjected to statistical analysis using analysis of variance (ANOVA) and the Tukey-Kramer multiple comparison test. With the exception of Laboratory 3, none of the other laboratories was successful in recovering yeasts on all the media. Six of the media proved inadequate for the enumeration of yeasts in the mould invested environment and were therefore omitted from statistical analysis. No significant differences in quantitative data obtained on Rose-Bengal Chloramphenicol Agar (RBCA), Dichloran Rose-Bengal Chloramphenicol Agar (DRBC), Dichloran 18% Glycerol Agar (DG18), and Malt extract agar supplemented with NaCl and oxytetracycline (MES) were detected by four of the collaborating laboratories whereas one laboratory found RBCA to be superior for yeast enumeration. DG18 and Malt Extract Agar with Biphenyl (MEB), however, were ranked superior based on qualitative results compared to the other media, attributed to distinctive individual yeast colonies and mould inhibition. RBCA, DRBC, DG18, and MES on the other hand, all proved to be adequate in supporting yeast colony development for quantitative analysis in samples obtained from blue veined cheeses.

Original languageEnglish
Pages (from-to)9-14
Number of pages6
JournalInternational Journal of Food Microbiology
Volume94
Issue number1
DOIs
Publication statusPublished - Jul 1 2004

Fingerprint

selective media
Cheese
Agar
cheeses
Rose Bengal
Yeasts
agar
yeasts
Chloramphenicol
chloramphenicol
molds (fungi)
malt extract
Fungi
statistical analysis
Oxytetracycline
oxytetracycline
Growth
Glycerol
biphenyl
microbial growth

Keywords

  • Blue-veined cheese
  • Enumeration
  • Selective media
  • Statistical significance
  • Yeasts

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

An inter-laboratory evaluation of selective media for the detection and enumeration of yeasts from blue-veined cheese. / Viljoen, B. C.; Knox, A.; Beuchat, L. R.; Deák, T.; Malfeito-Ferreira, M.; Hansen, T. K.; Hugo, A.; Jakobsen, M.; Loureiro, V.; Lourens-Hattingh, A.; Vasdinnyei, R.

In: International Journal of Food Microbiology, Vol. 94, No. 1, 01.07.2004, p. 9-14.

Research output: Contribution to journalArticle

Viljoen, BC, Knox, A, Beuchat, LR, Deák, T, Malfeito-Ferreira, M, Hansen, TK, Hugo, A, Jakobsen, M, Loureiro, V, Lourens-Hattingh, A & Vasdinnyei, R 2004, 'An inter-laboratory evaluation of selective media for the detection and enumeration of yeasts from blue-veined cheese', International Journal of Food Microbiology, vol. 94, no. 1, pp. 9-14. https://doi.org/10.1016/j.ijfoodmicro.2003.09.013
Viljoen, B. C. ; Knox, A. ; Beuchat, L. R. ; Deák, T. ; Malfeito-Ferreira, M. ; Hansen, T. K. ; Hugo, A. ; Jakobsen, M. ; Loureiro, V. ; Lourens-Hattingh, A. ; Vasdinnyei, R. / An inter-laboratory evaluation of selective media for the detection and enumeration of yeasts from blue-veined cheese. In: International Journal of Food Microbiology. 2004 ; Vol. 94, No. 1. pp. 9-14.
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