An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling

Thiemo B. Schreiber, Nina Mäusbacher, G. Kéri, Jürgen Cox, Henrik Daub

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Lysophosphatidic acid (LPA) induces a variety of cellular signaling pathways through the activation of its cognate G protein-coupled receptors. To investigate early LPA responses and assess the contribution of epidermal growth factor (EGF) receptor transactivation in LPA signaling, we performed phosphoproteomics analyses of both total cell lysate and protein kinase-enriched fractions as complementary strategies to monitor phosphorylation changes in A498 kidney carcinoma cells. Our integrated work flow enabled the identification and quantification of more than 5,300 phosphorylation sites of which 224 were consistently regulated by LPA. In addition to induced phosphorylation events, we also obtained evidence for early dephosphorylation reactions due to rapid phosphatase regulation upon LPA treatment. Phosphorylation changes induced by direct heparin-binding EGF-like growth factor-mediated EGF receptor activation were typically weaker and only detected on a subset of LPA-regulated sites, indicating signal integration among EGF receptor transactivation and other LPA-triggered pathways. Our results reveal rapid phosphoregulation of many proteins not yet implicated in G protein-coupled receptor signaling and point to various additional mechanisms by which LPA might regulate cell survival and migration as well as gene transcription on the molecular level. Moreover, our phosphoproteomics analysis of both total lysate and kinase-enriched fractions provided highly complementary parts of the LPA-regulated signaling network and thus represents a useful and generic strategy toward comprehensive signaling studies on a system-wide level.

Original languageEnglish
Pages (from-to)1047-1062
Number of pages16
JournalMolecular and Cellular Proteomics
Volume9
Issue number6
DOIs
Publication statusPublished - Jun 2010

Fingerprint

Workflow
Phosphorylation
Epidermal Growth Factor Receptor
G-Protein-Coupled Receptors
Transcriptional Activation
Chemical activation
Cells
Cell signaling
lysophosphatidic acid
Transcription
Set theory
Phosphoric Monoester Hydrolases
Epidermal Growth Factor
Protein Kinases
Cell Movement
Heparin
Cell Survival
Intercellular Signaling Peptides and Proteins
Phosphotransferases
Genes

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Analytical Chemistry

Cite this

An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. / Schreiber, Thiemo B.; Mäusbacher, Nina; Kéri, G.; Cox, Jürgen; Daub, Henrik.

In: Molecular and Cellular Proteomics, Vol. 9, No. 6, 06.2010, p. 1047-1062.

Research output: Contribution to journalArticle

Schreiber, Thiemo B. ; Mäusbacher, Nina ; Kéri, G. ; Cox, Jürgen ; Daub, Henrik. / An integrated phosphoproteomics work flow reveals extensive network regulation in early lysophosphatidic acid signaling. In: Molecular and Cellular Proteomics. 2010 ; Vol. 9, No. 6. pp. 1047-1062.
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