An in vivo eyecup preparation for the rat

János Pálhalmi, Tamás Szikra, K. Kékesi, Andrea Papp, G. Juhász

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A method for the preparation of an in vivo eyecup and a complex stimulating-sampling device are described; these are suitable for long-term parallel neurochemical and electrophysiological experiments on the rat retina without any additives into the eyecup. In this in vivo eyecup the extracellular microenvironment is under the normal homeostatic control of the vascular system; no continuous exchange of the eyecup fluid and no addition of glutamate is necessary to maintain stable retinal electric responses and amino acid concentrations. The eyecup viability was tested by monitoring the electroretinogram (ERG) and the amino acid contents of the eyecup fluid sampled from the preretinal space by means of microdialysis. After the initial increase the b-wave of the ERG changed by less than 10% in maximal amplitude during experiments lasting 5 h. The glutamate, glutamine, and glycine levels proved comparatively, whereas the taurine level rose continuously throughout the experimental protocol. Recovery of ERG was achieved following exposure to bright background illumination. Total exchange of the eyecup volume requires 20 min at a flow rate of 1 μl/min. The effect of L-AP4 on the ERG was successfully reproduced, which suggests the applicability of this in vivo eyecup for pharmacological experiments on the rat retina.

Original languageEnglish
Pages (from-to)167-174
Number of pages8
JournalJournal of Neuroscience Methods
Volume105
Issue number2
DOIs
Publication statusPublished - Feb 15 2001

Fingerprint

Retina
Glutamic Acid
Amino Acids
Taurine
Microdialysis
Glutamine
Lighting
Glycine
Blood Vessels
Pharmacology
Equipment and Supplies
2-amino-4-phosphono-propinate

Keywords

  • Amino acids
  • b-wave
  • Electroretinogram (ERG)
  • Eyecup
  • In vivo
  • Microdialysis
  • Retina

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

An in vivo eyecup preparation for the rat. / Pálhalmi, János; Szikra, Tamás; Kékesi, K.; Papp, Andrea; Juhász, G.

In: Journal of Neuroscience Methods, Vol. 105, No. 2, 15.02.2001, p. 167-174.

Research output: Contribution to journalArticle

Pálhalmi, János ; Szikra, Tamás ; Kékesi, K. ; Papp, Andrea ; Juhász, G. / An in vivo eyecup preparation for the rat. In: Journal of Neuroscience Methods. 2001 ; Vol. 105, No. 2. pp. 167-174.
@article{16010e9f347f4d4ab76be466699815a3,
title = "An in vivo eyecup preparation for the rat",
abstract = "A method for the preparation of an in vivo eyecup and a complex stimulating-sampling device are described; these are suitable for long-term parallel neurochemical and electrophysiological experiments on the rat retina without any additives into the eyecup. In this in vivo eyecup the extracellular microenvironment is under the normal homeostatic control of the vascular system; no continuous exchange of the eyecup fluid and no addition of glutamate is necessary to maintain stable retinal electric responses and amino acid concentrations. The eyecup viability was tested by monitoring the electroretinogram (ERG) and the amino acid contents of the eyecup fluid sampled from the preretinal space by means of microdialysis. After the initial increase the b-wave of the ERG changed by less than 10{\%} in maximal amplitude during experiments lasting 5 h. The glutamate, glutamine, and glycine levels proved comparatively, whereas the taurine level rose continuously throughout the experimental protocol. Recovery of ERG was achieved following exposure to bright background illumination. Total exchange of the eyecup volume requires 20 min at a flow rate of 1 μl/min. The effect of L-AP4 on the ERG was successfully reproduced, which suggests the applicability of this in vivo eyecup for pharmacological experiments on the rat retina.",
keywords = "Amino acids, b-wave, Electroretinogram (ERG), Eyecup, In vivo, Microdialysis, Retina",
author = "J{\'a}nos P{\'a}lhalmi and Tam{\'a}s Szikra and K. K{\'e}kesi and Andrea Papp and G. Juh{\'a}sz",
year = "2001",
month = "2",
day = "15",
doi = "10.1016/S0165-0270(00)00361-7",
language = "English",
volume = "105",
pages = "167--174",
journal = "Journal of Neuroscience Methods",
issn = "0165-0270",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - An in vivo eyecup preparation for the rat

AU - Pálhalmi, János

AU - Szikra, Tamás

AU - Kékesi, K.

AU - Papp, Andrea

AU - Juhász, G.

PY - 2001/2/15

Y1 - 2001/2/15

N2 - A method for the preparation of an in vivo eyecup and a complex stimulating-sampling device are described; these are suitable for long-term parallel neurochemical and electrophysiological experiments on the rat retina without any additives into the eyecup. In this in vivo eyecup the extracellular microenvironment is under the normal homeostatic control of the vascular system; no continuous exchange of the eyecup fluid and no addition of glutamate is necessary to maintain stable retinal electric responses and amino acid concentrations. The eyecup viability was tested by monitoring the electroretinogram (ERG) and the amino acid contents of the eyecup fluid sampled from the preretinal space by means of microdialysis. After the initial increase the b-wave of the ERG changed by less than 10% in maximal amplitude during experiments lasting 5 h. The glutamate, glutamine, and glycine levels proved comparatively, whereas the taurine level rose continuously throughout the experimental protocol. Recovery of ERG was achieved following exposure to bright background illumination. Total exchange of the eyecup volume requires 20 min at a flow rate of 1 μl/min. The effect of L-AP4 on the ERG was successfully reproduced, which suggests the applicability of this in vivo eyecup for pharmacological experiments on the rat retina.

AB - A method for the preparation of an in vivo eyecup and a complex stimulating-sampling device are described; these are suitable for long-term parallel neurochemical and electrophysiological experiments on the rat retina without any additives into the eyecup. In this in vivo eyecup the extracellular microenvironment is under the normal homeostatic control of the vascular system; no continuous exchange of the eyecup fluid and no addition of glutamate is necessary to maintain stable retinal electric responses and amino acid concentrations. The eyecup viability was tested by monitoring the electroretinogram (ERG) and the amino acid contents of the eyecup fluid sampled from the preretinal space by means of microdialysis. After the initial increase the b-wave of the ERG changed by less than 10% in maximal amplitude during experiments lasting 5 h. The glutamate, glutamine, and glycine levels proved comparatively, whereas the taurine level rose continuously throughout the experimental protocol. Recovery of ERG was achieved following exposure to bright background illumination. Total exchange of the eyecup volume requires 20 min at a flow rate of 1 μl/min. The effect of L-AP4 on the ERG was successfully reproduced, which suggests the applicability of this in vivo eyecup for pharmacological experiments on the rat retina.

KW - Amino acids

KW - b-wave

KW - Electroretinogram (ERG)

KW - Eyecup

KW - In vivo

KW - Microdialysis

KW - Retina

UR - http://www.scopus.com/inward/record.url?scp=0035865029&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035865029&partnerID=8YFLogxK

U2 - 10.1016/S0165-0270(00)00361-7

DO - 10.1016/S0165-0270(00)00361-7

M3 - Article

C2 - 11275273

AN - SCOPUS:0035865029

VL - 105

SP - 167

EP - 174

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

SN - 0165-0270

IS - 2

ER -