An improved treatment procedure for testicular biopsy specimens offers more efficient sperm recovery: Case series

Zsolt P. Nagy, Paul Devroey, Greta Verheyen, André C. Van Steirteghem, Herman Tournaye

Research output: Contribution to journalArticle

47 Citations (Scopus)


Objective: To report an improved sperm recovery procedure from testicular biopsy specimens for intracytoplasmic sperm injection (ICSI). Design: Case series and controlled study. Setting: Procedures were performed in a tertiary IVF center. Patient(s): Nonobstructive azoospermic cases (15 patients) and obstructive azoospermic cases (5 patients). Intervention(s): Intracytoplasmic sperm injection was carried out using testicular sperm isolated from a testicular biopsy specimen either with or without erythrocyte lysing buffer treatment. Main Outcome Measure(s): The time required to collect spermatozoa and the intactness and fertilization and developmental rates of oocytes. Result(s): In 7 of the 15 nonobstructive cases, it was possible to perform ICSI when, after shredding of the testicular tissue, no (or virtually no) sperm were present. There was no difference in the fertilization rates (83% and 68%) and developmental rates (87% and 89%) of the 54 sibling oocytes from another 5 patients in whom ICSI was carried out with sperm either treated or not treated with erythrocyte lysing buffer. Conclusion(s): Erythrocyte lysing buffer treatment of testicular biopsy specimens enhances the efficiency of sperm collection in those cycles in which spermatozoa are present and does not affect fertilization and embryo development.

Original languageEnglish
Pages (from-to)376-379
Number of pages4
JournalFertility and Sterility
Issue number2
Publication statusPublished - Aug 1997



  • Assisted fertilization
  • Intracytoplasmic sperm injection
  • Male infertility
  • Microinjection
  • Nonobstructive azoospermia
  • Red-blood-cell lysis
  • Testicular biopsy
  • Testicular sperm recovery

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynaecology

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