An improved system to establish highly embryogenic haploid cell and protoplast cultures from pollen calluses of maize (Zea mays L.)

Guangyuan He, Jinrui Zhang, Kexiu Li, Zhiyong Xiong, Mingjie Chen, Junli Chang, Yuesheng Wang, Guangxiao Yang, B. Barnabás

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Regenerable, embryogenic haploid cell suspensions were initiated and established from type II pollen calluses of two selected Chinese maize genotypes (No 592 Y and 592.A2 LY). The induction frequency of friable, embryogenic callus (type II) was highly dependent on three factors: genotype, medium, cold pretreatment, and on their interactions. Repeated callus and cell selection during the culture procedure led to stable haploid suspensions consisting of fine clusters each containing 20-50 cells. The selected cell lines were able to maintain their morphogenic ability during long-term subculture (2 years). Protoplasts were successfully isolated from subcultured, friable, embryogenic pollen calluses and cultured on N6BM and N6K media using a feeder layer, obtained from 2-day-old suspension culture. Healthy plants were regenerated from protoplast-derived calluses.

Original languageEnglish
Pages (from-to)15-25
Number of pages11
JournalPlant Cell, Tissue and Organ Culture
Volume86
Issue number1
DOIs
Publication statusPublished - Jul 2006

Fingerprint

Protoplasts
Haploidy
Bony Callus
Pollen
haploidy
protoplasts
Zea mays
callus
Cell Culture Techniques
pollen
corn
Suspensions
cells
Genotype
Feeder Cells
genotype
varespladib methyl
cell suspension culture
pretreatment
cell lines

Keywords

  • Anther culture
  • Plant regeneration
  • Protoplast culture
  • Type II callus
  • Zea mays

ASJC Scopus subject areas

  • Plant Science
  • Biotechnology

Cite this

An improved system to establish highly embryogenic haploid cell and protoplast cultures from pollen calluses of maize (Zea mays L.). / He, Guangyuan; Zhang, Jinrui; Li, Kexiu; Xiong, Zhiyong; Chen, Mingjie; Chang, Junli; Wang, Yuesheng; Yang, Guangxiao; Barnabás, B.

In: Plant Cell, Tissue and Organ Culture, Vol. 86, No. 1, 07.2006, p. 15-25.

Research output: Contribution to journalArticle

He, Guangyuan ; Zhang, Jinrui ; Li, Kexiu ; Xiong, Zhiyong ; Chen, Mingjie ; Chang, Junli ; Wang, Yuesheng ; Yang, Guangxiao ; Barnabás, B. / An improved system to establish highly embryogenic haploid cell and protoplast cultures from pollen calluses of maize (Zea mays L.). In: Plant Cell, Tissue and Organ Culture. 2006 ; Vol. 86, No. 1. pp. 15-25.
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AU - He, Guangyuan

AU - Zhang, Jinrui

AU - Li, Kexiu

AU - Xiong, Zhiyong

AU - Chen, Mingjie

AU - Chang, Junli

AU - Wang, Yuesheng

AU - Yang, Guangxiao

AU - Barnabás, B.

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KW - Anther culture

KW - Plant regeneration

KW - Protoplast culture

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KW - Zea mays

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