An improved method was elaborated for the entrapment of Streptomyces iverini and Rhodococcus sp. cells into agar gel using organic solvents as gelation media. Criteria were for the organic solvents the relatively low water-solubility, medium high viscosity, density lower than 1 and 2<logP<4. 2-Ethyl-hexanol proved to be most suitable. The procedure was applied for the "batch" production of mithramycin and cholesterol oxidase.
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology