An improved method for Lowicryl K4M electron microscopic embedding of brain tissue

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Abstract

The Lowicryl K4M low-temperature embedding technique was modified for brain tissue to facilitate antigen localization in neuronal structures by using postembedding immunoelectron microscopy. Rat brains fixed by perfusion with a mixture of 1% glutaraldehyde and 1% paraformaldehyde, were sectioned into 200-μm-thick coronal sections, and the appropriate area of the sections further microdissected into small samples (200 × 700 × 700 μm). These samples were processed for K4M embedding using prolonged incubation times at each step. This resulted in a homogeneously infiltrated tissue with good preservation of ultrastructure and antigen reactivity. Despite the lack of osmium fixation, the morphology of the synapses was clearly identifiable.

Original languageEnglish
Pages (from-to)29-32
Number of pages4
JournalJournal of Neuroscience Methods
Volume30
Issue number1
DOIs
Publication statusPublished - Oct 1989

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Keywords

  • Electron microscopy
  • Lowicryl K4M
  • Neural tissue
  • Postembedding immunocytochemistry
  • Protein A-gold

ASJC Scopus subject areas

  • Neuroscience(all)

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