An immunocytochemical method for histamine detection

P. Kovacs, G. Csaba

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Abstract

An anti-histaminic immune serum, prepared in New Zealand male rabbits by immunization with a chemospecific antigen (diazotized para-amino-benzoyl-azohistamine), was labeled with fluorescein isothiocyanate for the purpose of histamine detection or was employed for demonstration of histamine using fluorescein-labeled protein A. The IgG fraction of the hyperimmune serum was isolated, purified, and tested for specificity by binding to 3H-histamine or to histamine-containing or histamine-treated cells. Histological preparations used for detection of histamine should be fixed preferably in Carnoy solution. The procedure can also be employed for ultra-histochemical detection of histamine, using ferritin-conjugated antibody or the peroxidase technique.

Original languageEnglish
Pages (from-to)50-58
Number of pages9
JournalActa Histochemica
Volume66
Issue number1
Publication statusPublished - 1980

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ASJC Scopus subject areas

  • Cell Biology

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