An IgM antibody is a potent immunosuppressive agent that inhibits B cell proliferation

Maria E. Alonso, F. Uher, J. Gergely

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The results of this study demonstrate that the culture supernatant of lipopolysaccharide (LPS)-stimulated murine spleen cells is able to inhibit the growth of freshly isolated B lymphocytes. The inhibition is specific for B cells because the suppression of the LPS, Fc-fragment of human IgG, dextran sulfate, and anti-μ induced proliferation of B, but not the Concanavalin A response of T lymphocytes could be shown. The cells producing the inhibitor do not adhere to plastic, and are Thy-1 negative but surface Ig positive, i.e. they are B lymphocytes. The regulatory substance is heat resistant, sensitive to trypsin treatment, and has a high molecular weight of -1000 kDa. Moreover, it is specifically adsorbed on, and can be eluted from, anti-μ and anti-lg immunoaffinity columns. Thus, it seems to be an IgM antibody. Non-specific effects were excluded by the ineffectiveness of poly- and monoclonal IgM proteins. IgM-IgG complexes were also excluded. Thus, these results suggest the existence of a novel IgM antibody mediated control mechanism regulating B cell growth during polyclonal activation.

Original languageEnglish
Pages (from-to)1283-1288
Number of pages6
JournalInternational Immunology
Volume3
Issue number12
DOIs
Publication statusPublished - Dec 1991

Fingerprint

B Lymphocytes
Cell Proliferation
Lymphocytes
B Cells
Cell proliferation
Immunosuppressive Agents
Antibody
Antibodies
Immunoglobulin M
B-Lymphocytes
T Lymphocytes
T-cells
Dextran
Cell
Cell growth
Proliferation
Inhibitor
Lipopolysaccharides
Activation
Plastics

Keywords

  • B lymphocyte
  • IgM antibody
  • Immunosuppression
  • Lipopolysaccharide
  • Mouse

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation
  • Immunology

Cite this

An IgM antibody is a potent immunosuppressive agent that inhibits B cell proliferation. / Alonso, Maria E.; Uher, F.; Gergely, J.

In: International Immunology, Vol. 3, No. 12, 12.1991, p. 1283-1288.

Research output: Contribution to journalArticle

@article{7440faf963824b2186190a945dcf137f,
title = "An IgM antibody is a potent immunosuppressive agent that inhibits B cell proliferation",
abstract = "The results of this study demonstrate that the culture supernatant of lipopolysaccharide (LPS)-stimulated murine spleen cells is able to inhibit the growth of freshly isolated B lymphocytes. The inhibition is specific for B cells because the suppression of the LPS, Fc-fragment of human IgG, dextran sulfate, and anti-μ induced proliferation of B, but not the Concanavalin A response of T lymphocytes could be shown. The cells producing the inhibitor do not adhere to plastic, and are Thy-1 negative but surface Ig positive, i.e. they are B lymphocytes. The regulatory substance is heat resistant, sensitive to trypsin treatment, and has a high molecular weight of -1000 kDa. Moreover, it is specifically adsorbed on, and can be eluted from, anti-μ and anti-lg immunoaffinity columns. Thus, it seems to be an IgM antibody. Non-specific effects were excluded by the ineffectiveness of poly- and monoclonal IgM proteins. IgM-IgG complexes were also excluded. Thus, these results suggest the existence of a novel IgM antibody mediated control mechanism regulating B cell growth during polyclonal activation.",
keywords = "B lymphocyte, IgM antibody, Immunosuppression, Lipopolysaccharide, Mouse",
author = "Alonso, {Maria E.} and F. Uher and J. Gergely",
year = "1991",
month = "12",
doi = "10.1093/intimm/3.12.1283",
language = "English",
volume = "3",
pages = "1283--1288",
journal = "International Immunology",
issn = "0953-8178",
publisher = "Oxford University Press",
number = "12",

}

TY - JOUR

T1 - An IgM antibody is a potent immunosuppressive agent that inhibits B cell proliferation

AU - Alonso, Maria E.

AU - Uher, F.

AU - Gergely, J.

PY - 1991/12

Y1 - 1991/12

N2 - The results of this study demonstrate that the culture supernatant of lipopolysaccharide (LPS)-stimulated murine spleen cells is able to inhibit the growth of freshly isolated B lymphocytes. The inhibition is specific for B cells because the suppression of the LPS, Fc-fragment of human IgG, dextran sulfate, and anti-μ induced proliferation of B, but not the Concanavalin A response of T lymphocytes could be shown. The cells producing the inhibitor do not adhere to plastic, and are Thy-1 negative but surface Ig positive, i.e. they are B lymphocytes. The regulatory substance is heat resistant, sensitive to trypsin treatment, and has a high molecular weight of -1000 kDa. Moreover, it is specifically adsorbed on, and can be eluted from, anti-μ and anti-lg immunoaffinity columns. Thus, it seems to be an IgM antibody. Non-specific effects were excluded by the ineffectiveness of poly- and monoclonal IgM proteins. IgM-IgG complexes were also excluded. Thus, these results suggest the existence of a novel IgM antibody mediated control mechanism regulating B cell growth during polyclonal activation.

AB - The results of this study demonstrate that the culture supernatant of lipopolysaccharide (LPS)-stimulated murine spleen cells is able to inhibit the growth of freshly isolated B lymphocytes. The inhibition is specific for B cells because the suppression of the LPS, Fc-fragment of human IgG, dextran sulfate, and anti-μ induced proliferation of B, but not the Concanavalin A response of T lymphocytes could be shown. The cells producing the inhibitor do not adhere to plastic, and are Thy-1 negative but surface Ig positive, i.e. they are B lymphocytes. The regulatory substance is heat resistant, sensitive to trypsin treatment, and has a high molecular weight of -1000 kDa. Moreover, it is specifically adsorbed on, and can be eluted from, anti-μ and anti-lg immunoaffinity columns. Thus, it seems to be an IgM antibody. Non-specific effects were excluded by the ineffectiveness of poly- and monoclonal IgM proteins. IgM-IgG complexes were also excluded. Thus, these results suggest the existence of a novel IgM antibody mediated control mechanism regulating B cell growth during polyclonal activation.

KW - B lymphocyte

KW - IgM antibody

KW - Immunosuppression

KW - Lipopolysaccharide

KW - Mouse

UR - http://www.scopus.com/inward/record.url?scp=0026348405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026348405&partnerID=8YFLogxK

U2 - 10.1093/intimm/3.12.1283

DO - 10.1093/intimm/3.12.1283

M3 - Article

VL - 3

SP - 1283

EP - 1288

JO - International Immunology

JF - International Immunology

SN - 0953-8178

IS - 12

ER -