An evaluation of microwave heating for the rapid hydrolysis of peptide samples for chiral amino acid analysis.

A. Péter, G. Laus, D. Tourwé, E. Gerlo, G. Van Binst

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The hydrolysis of peptide samples in 6 N deuterium chloride by microwave heating has been investigated. The influence of the power and time of heating on the recovery of the amino acids and on their racemization was studied. A high recovery of sensitive amino acids and minimal racemization, as determined by the amount of deuterium incorporation, is obtained by using an intermediate irradiation power and 30 min reaction time.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalPeptide Research
Volume6
Issue number1
Publication statusPublished - Jan 1993

Fingerprint

Microwave heating
Deuterium
Microwaves
Heating
Hydrolysis
Amino Acids
Recovery
Peptides
Chlorides
Irradiation

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

Cite this

An evaluation of microwave heating for the rapid hydrolysis of peptide samples for chiral amino acid analysis. / Péter, A.; Laus, G.; Tourwé, D.; Gerlo, E.; Van Binst, G.

In: Peptide Research, Vol. 6, No. 1, 01.1993, p. 48-52.

Research output: Contribution to journalArticle

Péter, A. ; Laus, G. ; Tourwé, D. ; Gerlo, E. ; Van Binst, G. / An evaluation of microwave heating for the rapid hydrolysis of peptide samples for chiral amino acid analysis. In: Peptide Research. 1993 ; Vol. 6, No. 1. pp. 48-52.
@article{b163d50411eb4a67a0b43d6805ba64fb,
title = "An evaluation of microwave heating for the rapid hydrolysis of peptide samples for chiral amino acid analysis.",
abstract = "The hydrolysis of peptide samples in 6 N deuterium chloride by microwave heating has been investigated. The influence of the power and time of heating on the recovery of the amino acids and on their racemization was studied. A high recovery of sensitive amino acids and minimal racemization, as determined by the amount of deuterium incorporation, is obtained by using an intermediate irradiation power and 30 min reaction time.",
author = "A. P{\'e}ter and G. Laus and D. Tourw{\'e} and E. Gerlo and {Van Binst}, G.",
year = "1993",
month = "1",
language = "English",
volume = "6",
pages = "48--52",
journal = "Peptide Research",
issn = "1040-5704",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - An evaluation of microwave heating for the rapid hydrolysis of peptide samples for chiral amino acid analysis.

AU - Péter, A.

AU - Laus, G.

AU - Tourwé, D.

AU - Gerlo, E.

AU - Van Binst, G.

PY - 1993/1

Y1 - 1993/1

N2 - The hydrolysis of peptide samples in 6 N deuterium chloride by microwave heating has been investigated. The influence of the power and time of heating on the recovery of the amino acids and on their racemization was studied. A high recovery of sensitive amino acids and minimal racemization, as determined by the amount of deuterium incorporation, is obtained by using an intermediate irradiation power and 30 min reaction time.

AB - The hydrolysis of peptide samples in 6 N deuterium chloride by microwave heating has been investigated. The influence of the power and time of heating on the recovery of the amino acids and on their racemization was studied. A high recovery of sensitive amino acids and minimal racemization, as determined by the amount of deuterium incorporation, is obtained by using an intermediate irradiation power and 30 min reaction time.

UR - http://www.scopus.com/inward/record.url?scp=0027344658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027344658&partnerID=8YFLogxK

M3 - Article

VL - 6

SP - 48

EP - 52

JO - Peptide Research

JF - Peptide Research

SN - 1040-5704

IS - 1

ER -