An evaluation of microwave heating for the rapid hydrolysis of peptide samples for chiral amino acid analysis.

A. Péter, G. Laus, D. Tourwé, E. Gerlo, G. Van Binst

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The hydrolysis of peptide samples in 6 N deuterium chloride by microwave heating has been investigated. The influence of the power and time of heating on the recovery of the amino acids and on their racemization was studied. A high recovery of sensitive amino acids and minimal racemization, as determined by the amount of deuterium incorporation, is obtained by using an intermediate irradiation power and 30 min reaction time.

Original languageEnglish
Pages (from-to)48-52
Number of pages5
JournalPeptide research
Issue number1
Publication statusPublished - Jan 1 1993


ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology

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