An essential function of the mitochondrial sulfhydryl oxidase Erv1p/ALR in the maturation of cytosolic Fe/S proteins

Heike Lange, Thomas Lisowsky, Jana Gerber, Ulrich Mühlenhoff, G. Kispál, Roland Lill

Research output: Contribution to journalArticle

232 Citations (Scopus)

Abstract

Biogenesis of Fe/S clusters involves a number of essential mitochondrial proteins. Here, we identify the essential Erv1p of Saccharomyces cerevisia mitochondria as a novel component that is specifically required for the maturation of Fe/S proteins in the cytosol, but not in mitochondria. Furthermore, Erv1p was found to be important for cellular iron homeostasis. The homologous mammalian protein ALR ('augmenter of liver regeneration'), also termed hepatopoietin, can functionally replace defects in Erv1p and thus represents the mammalian orthologue of yeast Erv1p. Previously, a fragment of ALR was reported to exhibit an activity as an extracellular hepatotrophic growth factor. Both Erv1p and full-length ALR are located in the mitochondrial intermembrane space and represent the first components of this compartment with a role in the biogenesis of cytosolic Fe/S proteins. It is likely that Erv1p/ALR operates downstream of the mitochondrial ABC transporter Atm1p/ABC7/Sta1, which also executes a specific task in this essential biochemical process.

Original languageEnglish
Pages (from-to)715-720
Number of pages6
JournalEMBO Reports
Volume2
Issue number8
DOIs
Publication statusPublished - 2001

Fingerprint

Liver Regeneration
Protein S
Liver
Mitochondria
Yeast
Biochemical Phenomena
ATP-Binding Cassette Transporters
Hepatocyte Growth Factor
Mitochondrial Proteins
Cytosol
Saccharomyces cerevisiae
Intercellular Signaling Peptides and Proteins
Homeostasis
Iron
Yeasts
Defects
sulfhydryl oxidase
Proteins

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

An essential function of the mitochondrial sulfhydryl oxidase Erv1p/ALR in the maturation of cytosolic Fe/S proteins. / Lange, Heike; Lisowsky, Thomas; Gerber, Jana; Mühlenhoff, Ulrich; Kispál, G.; Lill, Roland.

In: EMBO Reports, Vol. 2, No. 8, 2001, p. 715-720.

Research output: Contribution to journalArticle

Lange, Heike ; Lisowsky, Thomas ; Gerber, Jana ; Mühlenhoff, Ulrich ; Kispál, G. ; Lill, Roland. / An essential function of the mitochondrial sulfhydryl oxidase Erv1p/ALR in the maturation of cytosolic Fe/S proteins. In: EMBO Reports. 2001 ; Vol. 2, No. 8. pp. 715-720.
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