An enzyme-linked immunosorbent assay (ELISA) for the detection of acetochlor

Gyöngyvér Hegedus, Vitalia Krikunova, Iván Bélai, Sergei Eremin, András Székács

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Competitive enzyme-linked immunosorbent assays (ELISAs) were developed in hapten-homologous and hapten-heterologous formats for the detection of the chloroacetanilide herbicide acetochlor. ELISA systems were devised using antibodies generated against acetochlor conjugated to carrier proteins through a thioether moiety replacing the chlorine atom in the parent structure, while haptens modified both on the chloroacetyl moiety and on the ethoxymethyl group of acetochlor have been used for coating antigens. The optimized ELISA systems allowed the detection of acetochlor 0.2-65 μg/L, and cross-reactivity studies revealed high specificity of the immunoassay: only four (propisochlor, butachlor, alachlor and metolachlor) among 18 structurally related acetanilide herbicides, fungicides and intermediates showed significant (> 1%) cross-reactivity, with even the highest value (propisochlor) being below 10%. Assay performance was not affected detrimentally by methanol up to 10% (v/v) and ethanol up to 5% (v/v). Assay performance was tested by measuring acetochlor concentration in water samples and compared favorably (r2=0.976) with those detected by gas chromatographic method coupled with mass spectrometry (GC-MS) using solid-phase microextraction (SPME) for sample preparation.

Original languageEnglish
Pages (from-to)879-891
Number of pages13
JournalInternational Journal of Environmental Analytical Chemistry
Volume82
Issue number11-12
DOIs
Publication statusPublished - Dec 1 2002

Keywords

  • Acetochlor
  • Enyzme immunoassay
  • Optimization
  • Sensitivity

ASJC Scopus subject areas

  • Analytical Chemistry
  • Environmental Chemistry
  • Water Science and Technology
  • Waste Management and Disposal
  • Soil Science
  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis

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