An electrospray mass spectrometric method for accurate mass determination of highly acid-sensitive phosphoramidites

Zoltán Kupihár, Zoltán Timár, Z. Darula, Douglas J. Dellinger, Marvin H. Caruthers

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

An accurate mass determination method utilizing electrospray ionization mass spectrometry is described for analysis of several different types of phosphoramidites that are extremely acid-sensitive compounds. An earlier method, which applied a LiCl/acetonitrile system, was extended for this special application by using polymeric standards including poly(ethylene glycol) (PEG), poly(ethylene glycol) dimethyl ether (PDE) and poly(propylene glycol) (PPG). Concentrations of standards, samples and LiCl were optimized and potential impurities that affect the analyses were also investigated.

Original languageEnglish
Pages (from-to)533-540
Number of pages8
JournalRapid Communications in Mass Spectrometry
Volume22
Issue number4
DOIs
Publication statusPublished - 2008

Fingerprint

Propylene Glycol
Acids
Electrospray ionization
Ethylene Glycol
Polyethylene glycols
Mass spectrometry
Impurities
phosphoramidite

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy

Cite this

An electrospray mass spectrometric method for accurate mass determination of highly acid-sensitive phosphoramidites. / Kupihár, Zoltán; Timár, Zoltán; Darula, Z.; Dellinger, Douglas J.; Caruthers, Marvin H.

In: Rapid Communications in Mass Spectrometry, Vol. 22, No. 4, 2008, p. 533-540.

Research output: Contribution to journalArticle

Kupihár, Zoltán ; Timár, Zoltán ; Darula, Z. ; Dellinger, Douglas J. ; Caruthers, Marvin H. / An electrospray mass spectrometric method for accurate mass determination of highly acid-sensitive phosphoramidites. In: Rapid Communications in Mass Spectrometry. 2008 ; Vol. 22, No. 4. pp. 533-540.
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