An electron spin resonance (ESR) study of free radicals formed from hematoxylin on tissue sections after blueing (alkalization)

G. Elek, A. Rockenbauer, L. Jókay

Research output: Contribution to journalArticle

Abstract

A free radical signal of 12 G width and g=2.0045 can be observed in hematoxylin stained tissue blocks and sections. The amount of paramagnetic centres in stained specimens is significantly larger than in unstained ones. After alkalization simultaneously with the colour change the former free radical is detectable in hemalum precipitate and on stained paper strips. After solution of the stain in dioxane and alkalization, a well resolved hyperfine structure could be seen which could be assigned to three different radicals with the same g value as observed in the rigid matrix (tissue and paper). Quantitative evaluation of free radical concentration is also carried out for tissue sections.

Original languageEnglish
Pages (from-to)137-140
Number of pages4
JournalHistochemistry
Volume92
Issue number2
DOIs
Publication statusPublished - Mar 1989

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Electron Spin Resonance Spectroscopy
Hematoxylin
Free Radicals
Coloring Agents
Color

ASJC Scopus subject areas

  • Anatomy

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An electron spin resonance (ESR) study of free radicals formed from hematoxylin on tissue sections after blueing (alkalization). / Elek, G.; Rockenbauer, A.; Jókay, L.

In: Histochemistry, Vol. 92, No. 2, 03.1989, p. 137-140.

Research output: Contribution to journalArticle

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