An Easy Method for Cryopreservation of Zebrafish (Danio rerio) Sperm

Caroline Caetano Da Silva, Tímea Kollár, Zsolt Csenki-Bakos, Bianca H.V. Fernandes, Akos Horvath, Luciani R. Carvalho

Research output: Contribution to journalArticle


We developed an easy, efficient, and cheap protocol for zebrafish sperm cryopreservation carried out on dry ice (20 min) using simple composition solution (200 mM glucose, 40 mM KCl, 30 mM Tris, pH = 8.0). The average efficiency of the present cryopreserve method was between 10% and 20% (expressed as fertilization rate). The experiments were conducted and repeated at two different locations, in different countries, yielding very similar results, showing the reproducibility and applicability of the method.

Original languageEnglish
Pages (from-to)321-323
Number of pages3
Issue number3
Publication statusPublished - Jun 2019


  • Danio rerio
  • cryopreservation
  • dry ice
  • sperm
  • zebrafish

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Developmental Biology

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  • Cite this

    Caetano Da Silva, C., Kollár, T., Csenki-Bakos, Z., Fernandes, B. H. V., Horvath, A., & Carvalho, L. R. (2019). An Easy Method for Cryopreservation of Zebrafish (Danio rerio) Sperm. Zebrafish, 16(3), 321-323.