An E-box motif conveys inhibitory activity on the atrial natriuretic peptide gene

M. Garami, David G. Gardner

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Atrial natriuretic peptide (ANP) is a potent diuretic, natriuretic, and vasorelaxant hormone that is expressed early in ventricular hypertrophy. Expression of human ANP is controlled by a series of regulatory elements located in the 5' flanking sequence of its gene. We generated a series of 5' deletion mutations extending from -2600 to -1150 relative to the transcription start site and linked them to a chloramphenicol acetyltransferase reporter gene. Using transient transfection analysis, we have identified a negative regulatory element between -1206 and -1152 relative to the start site. Each of a series of 5' deletion mutants, when introduced into fibroblast cultures, expressed the reporter function at a level that was significantly less (

Original languageEnglish
Pages (from-to)315-319
Number of pages5
JournalHypertension
Volume28
Issue number2
Publication statusPublished - Aug 1996

Fingerprint

E-Box Elements
Atrial Natriuretic Factor
Natriuretic Agents
Chloramphenicol O-Acetyltransferase
Sequence Deletion
5' Flanking Region
Transcription Initiation Site
Vasodilator Agents
Reporter Genes
Diuretics
Hypertrophy
Genes
Transfection
Fibroblasts

Keywords

  • gene expression
  • natriuretic peptides

ASJC Scopus subject areas

  • Internal Medicine

Cite this

An E-box motif conveys inhibitory activity on the atrial natriuretic peptide gene. / Garami, M.; Gardner, David G.

In: Hypertension, Vol. 28, No. 2, 08.1996, p. 315-319.

Research output: Contribution to journalArticle

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