An assay to detect in vivo y chromosome loss in drosophila wing disc cells

J. Szabad, Hugo J. Bellen, Koen J T Venken

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Loss of the Y chromosome in Drosophila has no impact on cell viability and therefore allows us to assay the impact of environmental agents and genetic alterations on chromosomal loss. To detect in vivo chromosome loss in cells of the developing Drosophila wing primordia, we first engineered a Y chromosome with an attP docking site. By making use of the FC31 integrase system, we site-specifically integrated a genomic transgene encompassing the multiple wing hair (mwh) locus into this attP site, leading to a mwh+Y chromosome. This chromosome fully rescues the mwh mutant phenotype, an excellent recessive wing cell marker mutation. Loss of this mwh+Y chromosome in wing primordial cells then leads to manifestation of the mwh mutant phenotype in mwh-homozygous cells. The forming mwh clones permit us to quantify the effect of agents and genetic alterations by assaying frequency and size of the mwh mosaic spots. To illustrate the use of the mwh+Y loss system, the effects of four known mutagens (X-rays, colchicine, ethyl methanesulfonate, and formaldehyde) and two genetic conditions (loss- and gain-of-function lodestar mutant alleles) are documented. The procedure is simple, sensitive, and inexpensive.

Original languageEnglish
Pages (from-to)1095-1102
Number of pages8
JournalG3: Genes, Genomes, Genetics
Volume2
Issue number9
DOIs
Publication statusPublished - Sep 2012

Fingerprint

Y Chromosome
Drosophila
Chromosomes
3-chloro-4-(dichloromethyl)-5-hydroxy-2(5H)-furanone
Ethyl Methanesulfonate
Phenotype
Integrases
Alopecia
Colchicine
Transgenes
Formaldehyde
Cell Survival
Clone Cells
Alleles
X-Rays
Mutation

Keywords

  • chromosome loss
  • Drosophila
  • Hair
  • Multiple wing
  • Spots
  • Wing mosaic
  • Y chromosome

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Genetics(clinical)

Cite this

An assay to detect in vivo y chromosome loss in drosophila wing disc cells. / Szabad, J.; Bellen, Hugo J.; Venken, Koen J T.

In: G3: Genes, Genomes, Genetics, Vol. 2, No. 9, 09.2012, p. 1095-1102.

Research output: Contribution to journalArticle

Szabad, J. ; Bellen, Hugo J. ; Venken, Koen J T. / An assay to detect in vivo y chromosome loss in drosophila wing disc cells. In: G3: Genes, Genomes, Genetics. 2012 ; Vol. 2, No. 9. pp. 1095-1102.
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