An Arabidopsis cytochrome b561 with trans-membrane ferrireductase capability

Alajos Bérczi, Dan Su, Han Asard

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Ascorbate-reducible cytochromes b561 (Cyts-b561) are a class of intrinsic trans-membrane proteins. Tonoplast Cyt-b561 (TCytb), one of the four Cyt-b561 isoforms in Arabidopsis was localized to the tonoplast. We demonstrate here that the optical spectra, EPR spectra and redox potentials of recombinant TCytb are similar to those of the well characterized bovine chromaffin granule Cyt-b561. We provide evidence for the reduction of ferric-chelates by the reduced TCytb. It is also shown that TCytb is capable of trans-membrane electron transport from intracellular ascorbate to extracellular ferric-chelates in yeast cells.

Original languageEnglish
Pages (from-to)1505-1508
Number of pages4
JournalFEBS letters
Volume581
Issue number7
DOIs
Publication statusPublished - Apr 3 2007

Keywords

  • Iron metabolism
  • Redox titration
  • Two-heme cytochrome b561
  • Vacuole
  • trans-Membrane electron transfer

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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