An Arabidopsis cyclin promoter region is active in transgenic maize plants

A. Peres, K. Nikovics, J. De Almeida-Engler, G. Engler, D. Inzé, A. Fehér, D. Dudits

Research output: Contribution to journalArticle

Abstract

We have produced transgenic maize carrying Arabidopsis cyclin promoter and β-glucuronidase chimeric fusion (cycB1;1At::GUS) by direct DNA uptake into embryogenic cell suspension protoplasts to study the activity of this cyclin promoter in a heterologous cell system. The transgenic maize lines exhibiting different levels of reporter gene expression show that regulatory elements from cycB1;1At gene as a dicot promoter can function in maize. Expression patterns revealed by fluorimetric, as well as, histochemical GUS reporter enzymatic assays indicated cell division-dependent expression of the GUS gene driven by this cycB1;At;1 promoter with elevated activity in proliferating cells and meristems. Treatment of tissues with inhibitors of mitosis increased the activity of cycB1;1At promoter, that suggests a link between mitotic phase of cell division and activity of cycB1;1At regulatory elements in transgenic maize.

Original languageEnglish
Pages (from-to)223-230
Number of pages8
JournalCereal Research Communications
Volume27
Issue number3
Publication statusPublished - Jan 1 1999

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Keywords

  • Cell cycle
  • Cyclin promoter
  • Gene expression
  • Maize
  • Transgenic plants

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Genetics

Cite this

Peres, A., Nikovics, K., De Almeida-Engler, J., Engler, G., Inzé, D., Fehér, A., & Dudits, D. (1999). An Arabidopsis cyclin promoter region is active in transgenic maize plants. Cereal Research Communications, 27(3), 223-230.