An antibody-based construct carrying DNA-mimotope and targeting CR1(CD35) selectively suppresses human autoreactive B-lymphocytes

Elisaveta Voynova, Andrey Tchorbanov, Jozsef Prechl, Milena Nikolova, Marta Baleva, Anna Erdei, Tchavdar Vassilev

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

There is an urgent and unmet need for therapeutic agents targeting selectively disease-associated B-lymphocytes in autoantibody-mediated diseases. We have constructed a chimeric molecule able to cross-link cell surface immunoglobulin with the inhibitory complement receptor type 1 (CD35) on DNA-specific B cells from SLE (systemic lupus erythematosus) patients with the aim of selectively silencing them. This engineered molecule is made of copies of the DNA-mimotope peptide DWEYSVWLSN coupled to a monoclonal anti-CD35 antibody. We found that the DNA-like peptide chimera induced a dose-dependent decrease in the number of IgG anti-dsDNA antibody producing cells when PBMCs of lupus patients were cultured in its presence. Our data present evidence that clustering BCR and the inhibitory CR1 on disease-associated autoreactive B-lymphocytes selectively suppresses autoantibody production.

Original languageEnglish
Pages (from-to)168-173
Number of pages6
JournalImmunology letters
Volume116
Issue number2
DOIs
Publication statusPublished - Mar 15 2008

Keywords

  • Complement receptor 1 (CD35)
  • Inhibitory B cell receptors
  • SLE

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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