An alternative solid phase peptide fragment condensation protocol with improved efficiency

Nikolett Mihala, József Bódi, A. Gomory, Helga Süli-Vargha

Research output: Contribution to journalArticle

3 Citations (Scopus)


The success of solid phase peptide synthesis is often limited by the aggregation of the growing peptide chains on the resin. Working from the results of a study of model coupling reactions in solution between Z-Gly-Phe-OH and H-Phe-OBzl, we have achieved higher efficiency in the repetitive solid phase fragment condensation of VGVAPG, in a 3:1 chloroform-phenol solvent system, using diisopropylcarbodiimide (DIC) as coupling agent, and a combination of 3-hydroxy-3,4-dihydro-4-oxo-1,2,3-benzotriazine (HODhbt) and its tetrabutyl ammonium salt as additive, than in DMF with DIC and HODhbt alone.

Original languageEnglish
Pages (from-to)565-568
Number of pages4
JournalJournal of Peptide Science
Issue number10
Publication statusPublished - Nov 7 2001



  • Additives
  • Fragment condensation
  • Solid phase peptide synthesis
  • Solvent system

ASJC Scopus subject areas

  • Structural Biology
  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Organic Chemistry

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