An Agrobacterium-mediated transformation system for the tomato cultivar Kecskeméti 262

Z. Szabó, Z. Bánfalvi

Research output: Contribution to journalArticle

Abstract

Cotyledon explants of tomato (Lycopersicum esculentum Mill.) cv. Kecskeméti 262 were excised from 9-day-old in vitro seedlings. The transformation method of Arrillaga et al. (1998) was tested and found to be effective for the introduction of foreign genes into this variety. Using an Agrobacterium strain carrying the plasmid 35S GUS INT, ten independent transgenic lines retaining all the phenotypic markers of cv. Kecskeméti 262 were isolated on regeneration medium containing kanamycin and were tested further for β- glucuronidase activity. The results show that the method of Arrillaga et al. (1998) is suitable for the introduction of agronomically important genes into the cultivar Kecskeméti 262.

Original languageEnglish
Pages (from-to)221-226
Number of pages6
JournalActa Agronomica Hungarica
Volume48
Issue number3
DOIs
Publication statusPublished - 2000

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Agrobacterium
tomatoes
cultivars
kanamycin
cotyledons
plasmids
explants
genes
genetically modified organisms
seedlings
methodology

Keywords

  • Somatic variation
  • Tomato
  • Transformation

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

An Agrobacterium-mediated transformation system for the tomato cultivar Kecskeméti 262. / Szabó, Z.; Bánfalvi, Z.

In: Acta Agronomica Hungarica, Vol. 48, No. 3, 2000, p. 221-226.

Research output: Contribution to journalArticle

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