An adenovirus recombinant that expresses the human cytomegalovirus major envelope glycoprotein and induces neutralizing antibodies

Gary S. Marshall, Robert P. Ricciardi, Robert F. Rando, Jennifer Puck, Ruowen Ge, Stanley A. Plotkin, E. Gönczöl

Research output: Contribution to journalArticle

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Abstract

The gene of the human cytomegalovirus (HCMV) major envelope glycoprotein, gB, was cloned from the Towne strain and inserted into adenovirus type 5 downstream of the E3 promoter. The recombinant virus, Ad-gB, expressed antigenically related proteins of 58, 30, 25, and 23 kDa in A549 and MRC-5 cells; the 58-kDa protein had the same mobility as the native gB from HCMV-infected MRC-5 cells and virions. All four proteins were detected by a monospecific polyclonal antiserum and by a monoclonal antibody in immunoblot and immunofluorescence assays. Hamsters infected intranasally with live Ad-gB developed protein-specific and HCMV-neutralizing antibody. This study confirms the importance of gB in the generation of the neutralizing immune response to HCMV and demonstrates the potential of live adenoviruses as vaccine vectors.

Original languageEnglish
Pages (from-to)1177-1181
Number of pages5
JournalJournal of Infectious Diseases
Volume162
Issue number5
Publication statusPublished - Nov 1990

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Neutralizing Antibodies
Cytomegalovirus
Adenoviridae
Glycoproteins
Proteins
Adenovirus Vaccines
Cricetinae
Virion
Fluorescent Antibody Technique
Immune Sera
Monoclonal Antibodies
Viruses
Genes

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Immunology

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An adenovirus recombinant that expresses the human cytomegalovirus major envelope glycoprotein and induces neutralizing antibodies. / Marshall, Gary S.; Ricciardi, Robert P.; Rando, Robert F.; Puck, Jennifer; Ge, Ruowen; Plotkin, Stanley A.; Gönczöl, E.

In: Journal of Infectious Diseases, Vol. 162, No. 5, 11.1990, p. 1177-1181.

Research output: Contribution to journalArticle

Marshall, Gary S. ; Ricciardi, Robert P. ; Rando, Robert F. ; Puck, Jennifer ; Ge, Ruowen ; Plotkin, Stanley A. ; Gönczöl, E. / An adenovirus recombinant that expresses the human cytomegalovirus major envelope glycoprotein and induces neutralizing antibodies. In: Journal of Infectious Diseases. 1990 ; Vol. 162, No. 5. pp. 1177-1181.
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