An adenovirus recombinant that expresses the human cytomegalovirus major envelope glycoprotein and induces neutralizing antibodies

Gary S. Marshall, Robert P. Ricciardi, Robert F. Rando, Jennifer Puck, Ruowen Ge, Stanley A. Plotkin, Eva GÖnczÖl

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The gene ofthe human cytomegalovirus (HCMV) major envelope glycoprotein, gB, was cloned from the Towne strain and inserted into adenovirus type 5 downstream of the E3 promoter. The recombinant virus, Ad-gB, expressed antigenically related proteins of 58, 30, 25, and 23 kDa in AS49 and MRC-5 cells; the 58-kDa protein had the same mobility as the native gB from HCMV-infected MRC-5 cells and virions. All four proteins were detected by a monospecific polyclonal antiserum and by a monoclonal antibody in immunoblot and immunofluorescence assays. Hamsters infected intranasally with live Ad-gB developed protein-specific and HCMV-neutralizing antibody. This study confirms the importance ofgB in the generation ofthe neutralizing immune response to HCMV and demonstrates the potential of live adenoviruses as vaccine vectors.

Original languageEnglish
Pages (from-to)1177-1181
Number of pages5
JournalJournal of Infectious Diseases
Volume162
Issue number5
DOIs
Publication statusPublished - Nov 1990

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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