Amyloid-like fibril formation by trypsin in aqueous ethanol. inhibition of fibrillation by PEG

M. Kotormán, L. Mária Simon, Attila Borics, Márton Richárd Szabó, Kitti Szabó, Titanilla Szögi, L. Fülöp

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The formation of amyloid-like fibrils was studied by using the well-known serine protease trypsin as a model protein in the presence of ethanol as organic solvent. Trypsin forms amyloid-like fibrils in aqueous ethanol at pH = 7.0. The dye Congo red (CR) was used to detect the presence of amyloid-like fibrils in the samples. The binding of CR to fibrils led to an increase in absorption intensity and a red shift in the absorption band of CR. Thioflavin T (ThT) and 8-anilino-1-naphthalenesulfonic acid (ANS) binding assays were employed to characterize amyloid-like fibril formation. The ThT binding assay revealed that the protein exhibited maximum aggregation in 60% (v/v) ethanol after incubation for 24 h at 24 oC. The ANS binding results indicated that the hydrophobic residues were more exposed to the solvent in the aggregated form of the protein. The effects of polyethylene glycol (PEG) on the formation of amyloid-like fibrils was studied in vitro. The aggregation of trypsin was followed via the kinetics of aggregation, the far-UV circular dichroism (CD) and transmission electron microscopy (TEM) in the presence and absence of PEG. The CD measurements indicated that the protein aggregates have a cross-beta structure in 60% ethanol. TEM revealed that trypsin forms fibrils with a thread-like structure. The inhibitory effect of PEG on the aggregation of trypsin increased with rising PEG concentration. PEG therefore inhibits the formation of amyloid-like fibrils of trypsin in aqueous ethanol.

Original languageEnglish
Pages (from-to)1104-1110
Number of pages7
JournalProtein and Peptide Letters
Volume22
Issue number12
Publication statusPublished - Dec 1 2015

Fingerprint

Amyloid
Trypsin
Ethanol
Congo Red
Agglomeration
Circular Dichroism
Transmission Electron Microscopy
Assays
Transmission electron microscopy
Proteins
Serine Proteases
Organic solvents
Absorption spectra
Coloring Agents
Kinetics

Keywords

  • Amyloid-like fibril
  • Circular dichroism
  • Congo red
  • Organic solvent
  • Polyethylene glycol
  • Trypsin

ASJC Scopus subject areas

  • Biochemistry
  • Structural Biology

Cite this

Amyloid-like fibril formation by trypsin in aqueous ethanol. inhibition of fibrillation by PEG. / Kotormán, M.; Simon, L. Mária; Borics, Attila; Szabó, Márton Richárd; Szabó, Kitti; Szögi, Titanilla; Fülöp, L.

In: Protein and Peptide Letters, Vol. 22, No. 12, 01.12.2015, p. 1104-1110.

Research output: Contribution to journalArticle

Kotormán, M, Simon, LM, Borics, A, Szabó, MR, Szabó, K, Szögi, T & Fülöp, L 2015, 'Amyloid-like fibril formation by trypsin in aqueous ethanol. inhibition of fibrillation by PEG', Protein and Peptide Letters, vol. 22, no. 12, pp. 1104-1110.
Kotormán M, Simon LM, Borics A, Szabó MR, Szabó K, Szögi T et al. Amyloid-like fibril formation by trypsin in aqueous ethanol. inhibition of fibrillation by PEG. Protein and Peptide Letters. 2015 Dec 1;22(12):1104-1110.
Kotormán, M. ; Simon, L. Mária ; Borics, Attila ; Szabó, Márton Richárd ; Szabó, Kitti ; Szögi, Titanilla ; Fülöp, L. / Amyloid-like fibril formation by trypsin in aqueous ethanol. inhibition of fibrillation by PEG. In: Protein and Peptide Letters. 2015 ; Vol. 22, No. 12. pp. 1104-1110.
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