Amylin is a novel neuropeptide with potential maternal functions in the rat

Éva Rebeka Szabó, Melinda Cservenák, A. Dobolyi

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Amylin, a 37-aa pancreatic peptide, was found to be expressed in the preoptic area of mother rats in our recent microarray study. Here, we report a marked increase in amylin expression around parturition and show that amylin mRNA level remains elevated as long as the pups are not removed from the dams. Amylin expression is also induced in maternally behaving (sensitized) nonlactating but not in nonsensitized nulliparous females or in females that did not become maternal despite the sensitization procedure. Immunohistochemistry verified the increased amylin peptide expression in maternally behaving rats and demonstrated the same expression pattern of amylin as in situ hybridization histochemistry. Ovariectomy had no effect on the activation of amylin neurons, suggesting sexual steroid-independent mechanisms. In subsequent functional experiments, mothers were separated from their pups for 22 h. On return of the pups, neuronal activation was found in the mother's preoptic area, with a distribution pattern similar to amylin-expressing neurons. Subsequent double labeling revealed that 86-93% of amylin neurons were activated by pup exposure. The results implicate amylin in the control of maternal adaptations, possibly exerting its actions on maternal behaviors via amylin receptors present in brain regions to which preoptic neurons project.

Original languageEnglish
Pages (from-to)272-281
Number of pages10
JournalFASEB Journal
Volume26
Issue number1
DOIs
Publication statusPublished - Jan 2012

Fingerprint

Islet Amyloid Polypeptide
Neuropeptides
Rats
Mothers
Neurons
Preoptic Area
Islet Amyloid Polypeptide Receptor
Chemical activation
Maternal Behavior
Peptides
Ovariectomy
Microarrays
Labeling
Dams
In Situ Hybridization
Brain
Immunohistochemistry
Steroids
Parturition

Keywords

  • Hypothalamus
  • Peptide expression
  • Postpartum mother
  • Preoptic area

ASJC Scopus subject areas

  • Biochemistry
  • Biotechnology
  • Genetics
  • Molecular Biology

Cite this

Amylin is a novel neuropeptide with potential maternal functions in the rat. / Szabó, Éva Rebeka; Cservenák, Melinda; Dobolyi, A.

In: FASEB Journal, Vol. 26, No. 1, 01.2012, p. 272-281.

Research output: Contribution to journalArticle

Szabó, Éva Rebeka ; Cservenák, Melinda ; Dobolyi, A. / Amylin is a novel neuropeptide with potential maternal functions in the rat. In: FASEB Journal. 2012 ; Vol. 26, No. 1. pp. 272-281.
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