Amygdala gene expression correlates of social behavior in monkeys experiencing maternal separation

Michael J. Sabatini, Philip Ebert, David A. Lewis, Pat Levitt, Judy L. Cameron, K. Mirnics

Research output: Contribution to journalArticle

82 Citations (Scopus)

Abstract

Children exposed to early parental loss from death or separation carry a greater risk for developing future psychiatric illnesses, such as major depression and anxiety. Monkeys experiencing maternal separation at 1 week of age show fewer social behaviors and an increase in self-comforting behaviors (e.g., thumb sucking) over development, whereas in contrast, monkeys experiencing maternal separation at 1 month of age show increased seeking of social comfort later in life. We sought to identify neural systems that may underlie these stress-induced behavioral changes by examining changes in mRNA content in amygdala tissue collected from 1 week separated, 1 month separated, and maternally reared infants at 3 months of age. mRNA from the right medial temporal lobe, primarily the amygdala, was analyzed using Affymetrix U133A 2.0 arrays. One gene, guanylate cyclase 1 α 3 (GUCY1A3), showed differential expression between the 1 week and maternally reared groups and the 1 week and 1 month groups; these changes were confirmed by in situ hybridization. The expression of this gene was positively correlated with acute social-comforting behavior (r = 0.923; p = 0.001) and longer-term close social behavior (r = 0.708; p = 0.015) and negatively correlated with self-comforting behaviors (r = -0.88; p <0.001). Additional in situ hybridization studies of GUCY1A3 in normal monkeys showed that this gene is expressed at adult levels by 1 week of age and that its expression is greater in the amygdala than all other brain areas examined. We conclude that GUCY1A3 may contribute to the altered behavioral phenotypes that are differentially displayed depending on the age at which macaque infants experience an early-life stress.

Original languageEnglish
Pages (from-to)3295-3304
Number of pages10
JournalJournal of Neuroscience
Volume27
Issue number12
DOIs
Publication statusPublished - Mar 21 2007

Fingerprint

Social Behavior
Amygdala
Haplorhini
Mothers
Gene Expression
In Situ Hybridization
Parental Death
Fingersucking
Messenger RNA
Macaca
Temporal Lobe
Psychological Stress
Genes
Psychiatry
Anxiety
Depression
Phenotype
Brain

Keywords

  • Amygdala
  • Behavior
  • DNA microarray
  • Gene expression
  • Guanylate cyclase
  • In situ hybridization
  • Maternal separation
  • Rhesus macaque

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Amygdala gene expression correlates of social behavior in monkeys experiencing maternal separation. / Sabatini, Michael J.; Ebert, Philip; Lewis, David A.; Levitt, Pat; Cameron, Judy L.; Mirnics, K.

In: Journal of Neuroscience, Vol. 27, No. 12, 21.03.2007, p. 3295-3304.

Research output: Contribution to journalArticle

Sabatini, Michael J. ; Ebert, Philip ; Lewis, David A. ; Levitt, Pat ; Cameron, Judy L. ; Mirnics, K. / Amygdala gene expression correlates of social behavior in monkeys experiencing maternal separation. In: Journal of Neuroscience. 2007 ; Vol. 27, No. 12. pp. 3295-3304.
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