Amplification of the phosphorylation site - ATP-binding site cDNA fragment of the Na+,K+-ATPase and the Ca2+-ATPase of Drosophila melanogaster by polymerase chain reaction

András Váradi, Maureen Gilmore-Heber, Edward J. Benz

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In vitro DNA-amplification technique has been utilized to generate a 430 bp fragment of the Na+,K+-ATPase, and a 550 bp fragment of a Ca2+-ATPase (the sarcoplasmic reticulum-type) of Drosophila melanogaster. The oligonucleotide primers for the DNA-amplification (Polymerase Chain Reaction) had been designed on the basis of amino acid sequence motifs - the phosphorylation site and the ATP-binding site - conserved among members of the ATPase protein family. Using the amplified cDNA-segments as probes, we demonstrated that there is one Na+,K+-ATPase and one Ca2+-ATPase (sarcoplasnaic reticulum-type) gene in the Drosophila genome. Three different mRNA species are processed from the Na+,K+-ATPase gene and one from the Ca2+-ATPase gene. Developmental control in expression of the Ca2+-ATPase gene was observed.

Original languageEnglish
Pages (from-to)203-207
Number of pages5
JournalFEBS letters
Volume258
Issue number2
DOIs
Publication statusPublished - Dec 4 1989

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Keywords

  • (Drosophila melanogaster)
  • ATPase
  • Developmental control of gene expression
  • Homology primer
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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