AMPA receptors colocalize with neuropeptide-Y- and galanin-containing, but not with dopamine, neurons of the female rat arcuate nucleus: A semiquantitative immunohistochemical colocalization study

Attila Zsarnovszky, Tamas L. Horvath, Frederick Naftolin, Csaba Leranth

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

It is well established that excitatory amino acid (EAA) neurotransmission is an essential component in the regulation of the gonadotropin-releasing hormone (GnRH) delivery system. However, the morphological interconnection of these systems is not fully understood. The objective of the present study was to determine whether or not alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptors - as indicators of aspartate/glutamatergic innervation - are present in the major neuronal populations, such as the neuropeptide-Y- (NPY), galanin- (GAL) and tyrosine-hydroxylase- (TH) containing neurons of the arcuate nucleus (AN) of the female rat. Colocalization experiments using the 'mirror' technique demonstrated that: (1) AN neurons containing GluR1 are also immunoreactive (IR) for GluR2/3; (2) 38.32% of AMPA-IR cells contain NPY and 31.72% of AMPA-containing neurons are also IR for GAL; in turn, 79.41% of NPY- and 56.19% of GAL-containing neurons are IR for AMPA receptors; none of the neurons are IR for both AMPA receptors and TH. These data suggest that an excitatory aspartate/glutamatergic input is implicated in the regulation of the examined neuropeptide-containing AN neurons but not in that of TH-IR cells of the same area.

Original languageEnglish
Pages (from-to)532-537
Number of pages6
JournalExperimental brain research
Volume133
Issue number4
DOIs
Publication statusPublished - Aug 19 2000

Keywords

  • Arcuate nucleus
  • Colocalization
  • Female rat
  • GluR
  • Immunostaining
  • Neuropeptides

ASJC Scopus subject areas

  • Neuroscience(all)

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