AMPA receptor blockade potentiates the stimulatory effect of L-DOPA on dopamine release in dopamine-deficient corticostriatal slice preparation

Zsolt Juranyi, Nora Sziray, Bernadett Marko, Gyorgy Levay, Laszlo G. Harsing

Research output: Contribution to journalReview article

10 Citations (Scopus)

Abstract

The release of [3H]dopamine was measured in rat corticostriatal slice preparations that contained the striatum and the adjacent prefrontal cortex to maintained glutamatergic corticostriatal afferentation. These slices were prepared from either nontreated or 6-hydroxydopamine-pretreated rats. The slices were loaded with [3H]dopamine, submerged in a two-compartment bath so that the cortical region was contained in one compartment, the corpus callosum was passed through a silicone greased slot, and the striatal region was contained in the other compartment. The cortical and the striatal parts were supervised with Krebs-bicarbonate buffer independently. The release of [ 3H]dopamine was determined from the striatal part at rest and in response to electrical stimulation of the cortical area. Electrical stimulation of the cortical part increased the release of [3H]dopamine from the striatal part of the slices, and this release was found to be higher after lesion of the nigrostriatal dopaminergic pathway with 6-hydroxydopamine. Cortically evoked [3H]dopamine release was even higher in the presence of the dopamine precursor L-DOPA after 6-hydroxdopamine lesion. Perfusion of GYKI-53405, a noncompetitive AMPA receptor antagonist, in combination with L-DOPA further increased both basal and stimulation-evoked [3H]dopamine release, whereas GYKi-53405 by itself did not influence basal [3H]dopamine outflow from striatum. These findings indicate that, in parkinsonian striatum, the stimulatory effect of L-DOPA on dopamine release is potentiated by AMPA receptor blockade, and the antiparkinsonian effect of GYKI-53405 may be due to its L-DOPA sparing effect.

Original languageEnglish
Pages (from-to)129-139
Number of pages11
JournalCritical Reviews in Neurobiology
Volume16
Issue number1-2
DOIs
Publication statusPublished - Dec 15 2004

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Keywords

  • 6-hydroxydopamine
  • Corticostriatal slice
  • GYKI-53405
  • L-DOPA
  • [H]dopamine release

ASJC Scopus subject areas

  • Neuroscience(all)
  • Physiology
  • Clinical Neurology
  • Physiology (medical)

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