Amoebapore homologs of Caenorhabditis elegans

Research output: Contribution to journalArticle

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Abstract

It is shown that the Caenorhabditis elegans genome contains several distantly related members of the gene family of saposin-like proteins. The putative products of genes T07C4.4, T08A9.7A, T08A9.7B, T08A9.8, T08A9.9, T08A9.10 are similar to the amoebapores of Entamoeba histolytica, granulysin of cytotoxic T lymphocytes and a putative amoebapore-related protein of the liver fluke Fasciola hepatica inasmuch as they consist of only a single saposin-like domain and a secretory signal peptide. The saposin-like domain of protein T07C4.4, which is most closely related to NK-lysin and granulysin, has been expressed in Escherichia coli and the recombinant protein was shown to have a circular dichroism spectrum consistent with the helix bundle structure characteristic of saposin-like domains. Recombinant T07C4.4 protein was found to have antibacterial activity, suggesting that these amoebapore homologs may play a role in antibacterial mechanisms of C. elegans. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)259-264
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Protein Structure and Molecular
Volume1429
Issue number1
DOIs
Publication statusPublished - Dec 8 1998

Fingerprint

Saposins
Caenorhabditis elegans
Fasciola hepatica
Genes
Recombinant Proteins
Entamoeba histolytica
Proteins
T-cells
Escherichia coli Proteins
Dichroism
Cytotoxic T-Lymphocytes
Protein Sorting Signals
Circular Dichroism
Liver
Escherichia coli
Genome
protozoan amoebapore proteins

Keywords

  • Amoebapore
  • Caenorhabditis elegans
  • Granulysin
  • NK-lysin
  • Saposin-like domain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Structural Biology
  • Biophysics

Cite this

Amoebapore homologs of Caenorhabditis elegans. / Bányai, L.; Patthy, L.

In: Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular, Vol. 1429, No. 1, 08.12.1998, p. 259-264.

Research output: Contribution to journalArticle

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